Application of the optical waveguide lightmode spectroscopy to monitor lipid bilayer phase transition

R. Horváth, G. Fricsovszky, E. Papp

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

An instrument for optical waveguide lightmode spectroscopy (OWLS) was designed and developed for measurements at different and controlled temperatures in a range of 15 °C around room temperature. The instrument allows to scan the waveguide modes at different wavelengths on the same optical chip using different lasers. This instrument was used to monitor DMPC lipid bilayer main phase transition around the critical temperature. The main problem in these experiments is that the OWLS measurements do not give enough information about an optically anisotropic system like a lipid bilayer. Experimental OWLS data at two different wavelengths can however approximately solve the problem. The temperature dependence of the thickness and the refractive indices (ordinary and extraordinary) for the lipid bilayer around the phase transition is presented. (A theoretical derivation of the extraordinary refractive index is given in Appendix A.)

Original languageEnglish
Pages (from-to)415-428
Number of pages14
JournalBiosensors and Bioelectronics
Volume18
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

Fingerprint

Lipid bilayers
Phase Transition
Lipid Bilayers
Optical waveguides
Spectrum Analysis
Phase transitions
Spectroscopy
Refractometry
Temperature
Refractive index
Dimyristoylphosphatidylcholine
Wavelength
Waveguides
Lasers
Experiments

Keywords

  • DMPC
  • Lipid bilayer
  • Optical waveguide lightmode spectroscopy
  • Phase transition

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Application of the optical waveguide lightmode spectroscopy to monitor lipid bilayer phase transition. / Horváth, R.; Fricsovszky, G.; Papp, E.

In: Biosensors and Bioelectronics, Vol. 18, No. 4, 01.04.2003, p. 415-428.

Research output: Contribution to journalArticle

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