Application of the immunoperoxidase technique to formalin fixed, paraffin embedded kidney biopsies.

E. Kemény, B. Iványi, A. Németh, S. Sonkodi

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5 Citations (Scopus)

Abstract

Different immunohistological methods were compared in 30 renal biopsy cases. The direct immunoperoxidase method gave identical results with immunofluorescence in 88% concerning IgG and C3, while IgA, IgM and fibrinogen have been identically detected by the PAP method in 82%. The non-identical results of the direct method consisted, with one exception, of negative readings, while the non-identical PAP readings were positive with two exceptions. Thus the trypsin-immunoperoxidase method proved to be well applicable on paraffin embedded formalin fixed material. In 10 cases the HRP-labelled direct method was applied also to fresh, frozen sections. It gave almost entirely identical results to immunofluorescence.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalZentralblatt für allgemeine Pathologie und pathologische Anatomie
Volume128
Issue number1-2
Publication statusPublished - 1983

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Immunoenzyme Techniques
Paraffin
Formaldehyde
Kidney
Biopsy
Fluorescent Antibody Technique
Reading
Frozen Sections
Trypsin
Fibrinogen
Immunoglobulin A
Immunoglobulin M
Immunoglobulin G

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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abstract = "Different immunohistological methods were compared in 30 renal biopsy cases. The direct immunoperoxidase method gave identical results with immunofluorescence in 88{\%} concerning IgG and C3, while IgA, IgM and fibrinogen have been identically detected by the PAP method in 82{\%}. The non-identical results of the direct method consisted, with one exception, of negative readings, while the non-identical PAP readings were positive with two exceptions. Thus the trypsin-immunoperoxidase method proved to be well applicable on paraffin embedded formalin fixed material. In 10 cases the HRP-labelled direct method was applied also to fresh, frozen sections. It gave almost entirely identical results to immunofluorescence.",
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AU - Kemény, E.

AU - Iványi, B.

AU - Németh, A.

AU - Sonkodi, S.

PY - 1983

Y1 - 1983

N2 - Different immunohistological methods were compared in 30 renal biopsy cases. The direct immunoperoxidase method gave identical results with immunofluorescence in 88% concerning IgG and C3, while IgA, IgM and fibrinogen have been identically detected by the PAP method in 82%. The non-identical results of the direct method consisted, with one exception, of negative readings, while the non-identical PAP readings were positive with two exceptions. Thus the trypsin-immunoperoxidase method proved to be well applicable on paraffin embedded formalin fixed material. In 10 cases the HRP-labelled direct method was applied also to fresh, frozen sections. It gave almost entirely identical results to immunofluorescence.

AB - Different immunohistological methods were compared in 30 renal biopsy cases. The direct immunoperoxidase method gave identical results with immunofluorescence in 88% concerning IgG and C3, while IgA, IgM and fibrinogen have been identically detected by the PAP method in 82%. The non-identical results of the direct method consisted, with one exception, of negative readings, while the non-identical PAP readings were positive with two exceptions. Thus the trypsin-immunoperoxidase method proved to be well applicable on paraffin embedded formalin fixed material. In 10 cases the HRP-labelled direct method was applied also to fresh, frozen sections. It gave almost entirely identical results to immunofluorescence.

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