Application of the dual-tagging gene trap method combined with a novel automatic selection system to identify genes involved in germ cell development in Drosophila melanogaster

P. Vilmos, L. Henn, M. Szathmári, T. Lukácsovich, L. Sipos, M. Erdélyi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The passage of highly specialized germ cells to future generations is essential for the maintenance of species. To date, conventional genetic screens identified relatively few genes that are involved in germ cell development. We aimed to identify germ line specific genes on the X chromosome of Drosophila melanogaster by the application of a new method: the dual-tagging gene-trap system (GT). A modified version of the gene-trap element was used in our experiments and the resulting insertional mutants were screened for grandchild-less phenotype with the help of the attached-X system and a sensitized genetic background developed in our laboratory. Among the 800 insertions mapped to the X chromosome 33 new mutations were identified that exhibited grandchild-less phenotype, 6 gave visible phenotype and 12 were conditional lethal. The cloning of a selected group of the 33 lines showing grandchild-less pheno-type confirmed that we have identified new candidates for genes involved in germ cell development. One of them named pebbled (peb) is discussed in details in this paper. Finally, we also describe a novel automatic selection system developed in our laboratory which enables the extension of the GT mutagenesis to the autosomes.

Original languageEnglish
Pages (from-to)81-94
Number of pages14
JournalActa Biologica Hungarica
Volume58
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Nov 2007

Fingerprint

germ cell
tagging
Drosophila melanogaster
Germ Cells
germ cells
traps
Genes
Cells
gene
phenotype
Phenotype
genes
X chromosome
Chromosomes
chromosome
X-Linked Genes
methodology
Social Responsibility
X Chromosome
Mutagenesis

Keywords

  • Drosophila melanogaster
  • Germ cell
  • Mutagenesis
  • Pebbled
  • SOT system

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Application of the dual-tagging gene trap method combined with a novel automatic selection system to identify genes involved in germ cell development in Drosophila melanogaster. / Vilmos, P.; Henn, L.; Szathmári, M.; Lukácsovich, T.; Sipos, L.; Erdélyi, M.

In: Acta Biologica Hungarica, Vol. 58, No. SUPPL. 1, 11.2007, p. 81-94.

Research output: Contribution to journalArticle

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