Application of sperm cryopreservation to hatchery practice and species conservation

A case of the Adriatic grayling (Thymallus thymallus)

A. Horváth, Dušan Jesenšek, Balázs Csorbai, Zoltán Bokor, Éva Raboczki, Dániel Kaczkó, Gergely Bernáth, György Hoitsy, B. Urbányi, Simona Sušnik Bajec, Aleš Snoj

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Sperm cryopreservation was successfully applied to the culture of the Adriatic grayling in the Soča river basin in Slovenia whose stocks have been affected by introgressive hybridization with non-native grayling from the Danubian drainage. Sperm was collected from wild males at the spawning sites and cryopreserved while fin clips of the same individuals were subjected to genetic analysis using molecular markers. Sperm samples from individuals having more than 95% of markers characteristicof the Adriatic genotype were thawed and used for fertilization of eggs from previously selected Adriatic grayling females. The hatched progeny is currently grown as a future broodstock or has been stocked into the wild. The present study presents the possibility of relatively short-term (2-6. weeks) storage of cryopreserved sperm as an alternative to long-term gene banking.

Original languageEnglish
Pages (from-to)213-215
Number of pages3
JournalAquaculture
Volume358-359
DOIs
Publication statusPublished - Aug 15 2012

Fingerprint

Thymallus
cryopreservation
species conservation
hatchery
sperm
hatcheries
spermatozoa
banking
breeding stock
Slovenia
genetic analysis
fertilization (reproduction)
introgression
fins
genetic techniques and protocols
drainage
spawning
genotype
river basin
egg

Keywords

  • Aquaculture
  • Conservation
  • Cryopreservation
  • Grayling
  • Sperm

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Application of sperm cryopreservation to hatchery practice and species conservation : A case of the Adriatic grayling (Thymallus thymallus). / Horváth, A.; Jesenšek, Dušan; Csorbai, Balázs; Bokor, Zoltán; Raboczki, Éva; Kaczkó, Dániel; Bernáth, Gergely; Hoitsy, György; Urbányi, B.; Bajec, Simona Sušnik; Snoj, Aleš.

In: Aquaculture, Vol. 358-359, 15.08.2012, p. 213-215.

Research output: Contribution to journalArticle

Horváth, A. ; Jesenšek, Dušan ; Csorbai, Balázs ; Bokor, Zoltán ; Raboczki, Éva ; Kaczkó, Dániel ; Bernáth, Gergely ; Hoitsy, György ; Urbányi, B. ; Bajec, Simona Sušnik ; Snoj, Aleš. / Application of sperm cryopreservation to hatchery practice and species conservation : A case of the Adriatic grayling (Thymallus thymallus). In: Aquaculture. 2012 ; Vol. 358-359. pp. 213-215.
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