Application of real-time RT-PCR utilising LUX (Light Upon Extension) fluorogenic primer for the rapid detection of avian influenza viruses

István Kiss, P. Germán, L. Sámi, Márta Antal, T. Farkas, G. Kardos, S. Kecskeméti, Á Dán, S. Belák

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A real-time RT-PCR assay utilising light upon extension fluorogenic primer (LUX RT-PCR) was developed for the rapid and efficient detection of avian influenza viruses (AIV). The assay detected each of the AIV isolates tested (16/16) and gave negative results with heterologous pathogens (17/17). The detection limit of the assay proved to be 10-0.5 EID50/0.2 ml and 101.5 EID50/0.2 ml in allantoic fluid of virus-infected embryonated chicken eggs and in spiked chicken faeces samples, respectively. Based on its specificity, sensitivity and relative simplicity, the LUX RT-PCR assay provides a novel, rapid and cost-effective diagnostic tool for avian influenza surveillance and monitoring programs.

Original languageEnglish
Pages (from-to)525-533
Number of pages9
JournalActa veterinaria Hungarica
Volume54
Issue number4
DOIs
Publication statusPublished - Nov 6 2006

Keywords

  • Avian influenza virus
  • LUX RT-PCR
  • Rapid detection

ASJC Scopus subject areas

  • veterinary(all)

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