Objectives: The feasibility of additional cytogenetic end-points (premature centromere division, PCD; premature chromosome condensation, PCC and satellite association, SA) was evaluated in peripheral blood lymphocyte metaphases routinely prepared for chromosome aberrations (CA) for genotoxicology monitoring and (cancer) risk assessment. Methods: Venous whole blood samples of400 Hungarians (188 controls, 155 occupationally exposed chemical industry workers and 57 cytostatic-drug-exposed hospital nurses) were routinely prepared using phytohemagglutinin stimulation, colchicine arrest in the 50th h, and Giemsa staining; 100 metaphases per donor were evaluated parallelly for PCD, PCC, 5/1 and CA. Results and conclusions: The increase in CA yields indicated the genotoxic exposure in each exposed groups. Significant increases of PCD and PCC yields were also observed in certain exposures, as well as in subgroups without adequate personal protection when compared to controls indicating exposure dependency. SA yields, however, were artificially high both in controls and in exposed suggesting that a methodical factor (probably colchicine) can be responsible for the high yields. Consequently, PCD (and probably PCC) can, while SA cannot be used for (cancer) risk assessment when the routine cytogenetic methods are in use.
|Translated title of the contribution||Application of premature centromere separation, satellite association, and premature chromosome condensation in the risk assessment of workers exposed to carcinogens|
|Number of pages||7|
|Publication status||Published - Dec 1 1999|
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