Application of polymerase chain reaction and virus isolation techniques for the detection of viruses in aborted and newborn foals

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Abstract

The occurrence of two important pathogens, equine herpesvirus 1 (EHV1) and equine arteritis virus (EAV) causing abortions, perinatal foal mortality and respiratory disease, was investigated by polymerase chain reaction (PCR) and virus isolation to demonstrate the presence of abortigenic viruses in samples from 248 horse fetuses in Hungary. We found 26 EHV1- and 4 EAV-positive aborted or prematurely born foals from 16 and 4 outbreaks, respectively, proving that despite the widely applied vaccination, EHV1 is a far more important cause of abortions in the studs than EAV. We compared the virus content of different organs of the fetuses by PCR and isolation to identify the organ most suitable for virus demonstration. Our investigations indicate that the quantity of both viruses is highest in the lungs; therefore, according to our observations, in positive cases the probability of detection is highest from lung samples of aborted or newborn foals. Both the PCR and the virus isolation results revealed that the liver, though widely used, is not the best organ to sample either for EHV1 or for EAV detection. From the analysis of the epidemiological data, we tried to estimate the importance of the two viruses in the Hungarian horse population.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalActa Veterinaria Hungarica
Volume54
Issue number2
DOIs
Publication statusPublished - 2006

Fingerprint

isolation techniques
foals
Equine Arteritis Virus
Equid Herpesvirus 1
Equine arteritis virus
neonates
polymerase chain reaction
Equid herpesvirus 1
Viruses
Polymerase Chain Reaction
viruses
abortion (animals)
Horses
fetus
Equid Herpesvirus 4
Fetus
lungs
Equid herpesvirus 4
horses
Lung

Keywords

  • Equine abortion
  • Equine arteritis virus
  • Equine herpesvirus
  • Equine rhinopneumonitis
  • Equine viral arteritis
  • PCR
  • Virus isolation

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Application of polymerase chain reaction and virus isolation techniques for the detection of viruses in aborted and newborn foals",
abstract = "The occurrence of two important pathogens, equine herpesvirus 1 (EHV1) and equine arteritis virus (EAV) causing abortions, perinatal foal mortality and respiratory disease, was investigated by polymerase chain reaction (PCR) and virus isolation to demonstrate the presence of abortigenic viruses in samples from 248 horse fetuses in Hungary. We found 26 EHV1- and 4 EAV-positive aborted or prematurely born foals from 16 and 4 outbreaks, respectively, proving that despite the widely applied vaccination, EHV1 is a far more important cause of abortions in the studs than EAV. We compared the virus content of different organs of the fetuses by PCR and isolation to identify the organ most suitable for virus demonstration. Our investigations indicate that the quantity of both viruses is highest in the lungs; therefore, according to our observations, in positive cases the probability of detection is highest from lung samples of aborted or newborn foals. Both the PCR and the virus isolation results revealed that the liver, though widely used, is not the best organ to sample either for EHV1 or for EAV detection. From the analysis of the epidemiological data, we tried to estimate the importance of the two viruses in the Hungarian horse population.",
keywords = "Equine abortion, Equine arteritis virus, Equine herpesvirus, Equine rhinopneumonitis, Equine viral arteritis, PCR, Virus isolation",
author = "A. Horny{\'a}k and T. Bakonyi and M{\'o}nika Kulik and S. Kecskem{\'e}ti and M. Rusvai",
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AU - Hornyák, A.

AU - Bakonyi, T.

AU - Kulik, Mónika

AU - Kecskeméti, S.

AU - Rusvai, M.

PY - 2006

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AB - The occurrence of two important pathogens, equine herpesvirus 1 (EHV1) and equine arteritis virus (EAV) causing abortions, perinatal foal mortality and respiratory disease, was investigated by polymerase chain reaction (PCR) and virus isolation to demonstrate the presence of abortigenic viruses in samples from 248 horse fetuses in Hungary. We found 26 EHV1- and 4 EAV-positive aborted or prematurely born foals from 16 and 4 outbreaks, respectively, proving that despite the widely applied vaccination, EHV1 is a far more important cause of abortions in the studs than EAV. We compared the virus content of different organs of the fetuses by PCR and isolation to identify the organ most suitable for virus demonstration. Our investigations indicate that the quantity of both viruses is highest in the lungs; therefore, according to our observations, in positive cases the probability of detection is highest from lung samples of aborted or newborn foals. Both the PCR and the virus isolation results revealed that the liver, though widely used, is not the best organ to sample either for EHV1 or for EAV detection. From the analysis of the epidemiological data, we tried to estimate the importance of the two viruses in the Hungarian horse population.

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KW - Equine arteritis virus

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