Application of pharmacia automated FPLC System and PepRPC HR 5/5 bonded phase column for determination of dansylated polyamines

T. Kremmer, M. Boldizsár, L. Holczinger

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The Pharmacia Automatized FPLC System equipped with a PepRPC HR 5/5 bonded-phase column was tested with dansyl polyamines, toluene, dimethyl- and dibutylphthalate, using either isocratic conditions or linear gradient-elution program of methanol in water as the mobile phase. A simple and reproducible method is described for the quantiation of dansylated natural polyamines, such as putrescine, spermidine and spermine originating from P388/S leukemia cells. Comparing the data from analyses performed in parallel by the Automated FPLC/PepRPC HR 5/5, and a Hewlett-Packard Model 1084B/LiChrosorb RP-8 systems a linear correlation has been found, with a regression coefficient of r=0.974.

Original languageEnglish
Pages (from-to)79-82
Number of pages4
JournalChromatographia
Volume20
Issue number2
DOIs
Publication statusPublished - Feb 1 1985

Keywords

  • Apolar test compounds
  • Automated fast-performance LC system
  • Dansyl polyamines, quantitation of
  • P388/S leukemia cells
  • Pep RPC HR 5/5 bonded phase column

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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