Application of overpressured layer chromatography combined with digital autoradiography and mass spectrometry in the study of deramciclane metabolism.

K. Ludányi, K. Vékey, J. Szúnyog, E. Mincsovics, T. Karancsi, K. Újszászy, K. B. Nemes, I. Klebovich

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Overpressured layer chromatography was combined with the highly sensitive and rapid digital autoradiography (DAR) and mass spectrometry to separate, detect, and identify 3H- and 14C-labeled deramciclane metabolites in different biological matrixes. Several minor and major metabolites were separated from plasma and urine samples. The radioactive metabolites localized by DAR were scraped from the thin-layer chromatographic plate and transferred to a mass spectrometer for structure identification. Several metabolites were isolated and characterized, including hydroxy-N-desmethyl deramciclane, which is described in detail. The combination of techniques is efficient and has good sensitivity: about 2 micrograms metabolite from a biological matrix was isolated and identified this way.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalJournal of AOAC International
Volume82
Issue number2
Publication statusPublished - Mar 1999

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autoradiography
Metabolites
Chromatography
Autoradiography
Metabolism
chromatography
Mass spectrometry
Mass Spectrometry
metabolite
mass spectrometry
metabolism
metabolites
Urine
matrix
Mass spectrometers
spectrometers
urine
spectrometer
deramciclane
Plasmas

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

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AU - Ludányi, K.

AU - Vékey, K.

AU - Szúnyog, J.

AU - Mincsovics, E.

AU - Karancsi, T.

AU - Újszászy, K.

AU - Nemes, K. B.

AU - Klebovich, I.

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