Application of OPLC-DAR and HPLC-RD techniques in metabolite research

B. Dalmadi Kiss, E. Mincsovics, K. Balogh Nemes, I. Klebovich

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Metabolite research in drug development is a complex analytical problem. Several chromatographic methods are used after sample extraction for the isolation and purification of metabolites from different biological matrices. This paper summarizes the combined use of overpressured layer chromatography with digital autoradiography (OPLC-DAR) and HPLC with radioactivity detection (HPLC-RD) for qualitative and quantitative determination of metabolites, depending on the complexity and radioactivity content of the matrix investigated.

Original languageEnglish
Pages (from-to)257-260
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Volume13
Issue number4
Publication statusPublished - Jan 1 2000

Keywords

  • Digital autoradiography (DAR)
  • HPLC-radioactivity detection (HPLC-RD)
  • Hyphenated techniques
  • Metabolism study
  • Overpressured layer chromatography (OPLC)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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