Application of in vitro lymphocyte stimulation test to investigate the immunoreactvity of sea bass (Dicentrarchus labrax)

M. Galeotti, D. Volpatti, L. D'Angelo, M. Rusvai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A rapid tetrazolium (MTT) based colorimetric assay was used to evaluate the in vitro cellular immune reactivity of sea bass (Dicentrarchus labrax) lymphocytes obtained from peripheral blood, head kidney and spleen, and separated on Hystopaque gradients. Dose response assays were performed using lectins (phytohemagglutinin, concanavalin-A) and bacterial lipopolysaccharide as stimulants. In the leukocyte microtitration assays the best results were observed in head kidney cell cultures maintained in the presence of PHA-P in 12.ìg/ml end concentration, but LPS also caused a definite proliferation, while Con-A proved to lack effect. Peripheral blood .5lymphocytes were stimulated by all three mitogens Con-A, LPS and PHA-P, the highest values were given also by PHA-P in cultures containing the drug in 6.25 ìg/ml concentration. Spleen lymphocytes reacted least to the mitogens, only mild, statistically non significant responses were observed in the presence of the superantigens.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalBulletin of the European Association of Fish Pathologists
Volume16
Issue number1
Publication statusPublished - 1996

Fingerprint

Bass
Dicentrarchus labrax
lymphocyte proliferation
concanavalin A
Lymphocyte Activation
bass
Head Kidney
assay
Mitogens
spleen
assays
lymphocytes
Spleen
blood
kidneys
superantigens
Lymphocytes
Superantigens
tetrazolium
phytohemagglutinin

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)

Cite this

Application of in vitro lymphocyte stimulation test to investigate the immunoreactvity of sea bass (Dicentrarchus labrax). / Galeotti, M.; Volpatti, D.; D'Angelo, L.; Rusvai, M.

In: Bulletin of the European Association of Fish Pathologists, Vol. 16, No. 1, 1996, p. 23-26.

Research output: Contribution to journalArticle

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