Application of HPLC-ICP-MS to speciation of cisplatin and its degradation products in water containing different chloride concentrations and in human urine

S. Hann, G. Koellensperger, Zs Stefánka, G. Stingeder, M. Fürhacker, W. Buchberger, R. M. Mader

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66 Citations (Scopus)

Abstract

Cisplatin, mono- and diaquacisplatin were measured in aquatic samples and in diluted urine of a cancer patient by HPLC-ICP-MS. On-line IDMS was applied for accurate, species unspecific quantification. Limits of detection of 0.74, 0.69 and 0.65 μg L-1 (3 s criterion) were calculated for cisplatin, monoaqua- and diaquacisplatin, respectively. Degradation kinetics of 6 × 10-6 M cisplatin were determined over a period of 48 h in solutions containing 100, 50 and 0 mg L-1 chloride, showing the suitability of the HPLC-ICP-MS method for kinetic model studies. The first order rate constants k1 of cisplatin aquation for the three chloride concentrations were 1.79 × 10-5, 1.68 × 10-5 and 2.06 × 10-5 s-1. For cisplatin anatation (second order reverse reaction), rate constants of k-1 = 6.5 × 10 -3, 5.8 × 10-3 and 4.1 × 10-3 M-1 s-1 could be assessed. At low chloride levels, no equilibrium was established between cisplatin and its degradation products. It was found that the intermediately formed mono- and diaquacisplatin-products started to decay after several hours. Diluted urine of a cancer patient contained the parent drug cisplatin and a considerable fraction of highly active monoaquacisplatin, as well as several unknown platinum species.

Original languageEnglish
Pages (from-to)1391-1395
Number of pages5
JournalJournal of analytical atomic spectrometry
Volume18
Issue number11
DOIs
Publication statusPublished - Dec 1 2003

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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