Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation

B. Sunil, K. Riazunnisa, T. Sai Krishna, G. Schansker, Reto J. Strasser, Agepati S. Raghavendra, Prasanna Mohanty

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Intact and metabolically very active mesophyll protoplasts were isolated rapidly from pea (Pisum sativum) leaves. The functional performance of protoplasts at various stages of their isolation was analyzed by using fast Chl a fluorescence OJIP transients and compared with that of intact leaves. The results demonstrated that the OJIP transients could successfully be used to monitor the quality of mesophyll protoplasts at different isolation steps. The protoplasts maintained their integrity and photosynthetic status very well, and their performance was very similar to that of the intact leaves.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalIndian Journal of Biochemistry and Biophysics
Volume45
Issue number1
Publication statusPublished - Feb 2008

Fingerprint

Protoplasts
Peas
Transient analysis
Fluorescence
chlorophyll a

Keywords

  • Chl a fluorescence
  • Functional integrity
  • Mesophyll protoplasts
  • O-J-I-P transients
  • Pisum sativum
  • Protoplast isolation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation. / Sunil, B.; Riazunnisa, K.; Sai Krishna, T.; Schansker, G.; Strasser, Reto J.; Raghavendra, Agepati S.; Mohanty, Prasanna.

In: Indian Journal of Biochemistry and Biophysics, Vol. 45, No. 1, 02.2008, p. 37-43.

Research output: Contribution to journalArticle

Sunil, B. ; Riazunnisa, K. ; Sai Krishna, T. ; Schansker, G. ; Strasser, Reto J. ; Raghavendra, Agepati S. ; Mohanty, Prasanna. / Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation. In: Indian Journal of Biochemistry and Biophysics. 2008 ; Vol. 45, No. 1. pp. 37-43.
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