Application of external micro-spectrophotometric detection to improve sensitivity on microchips

A. Gáspár, István Bácsi, Erika F. Garcia, M. Braun, Frank A. Gomez

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The goal of this work was to increase the sensitivity of a UV-Vis spectrophotometer by decreasing the background noise and lengthening the optical path. A microphotometer has been modified to precisely select very small parts of a microfluidic channel pattern of a chip and to measure light absorbance on a magnified area of the selected part of the channel. The viability of combining a projection microscope and a spectrophotometer for external absorbance measurements on disposable PDMS chips was studied. Besides the external direct detection above a microfluidic channel, the optical pathlength was lengthened by detecting in the region of the perpendicular exit port. Increasing the cross-sectional area of the zone of irradiation improved the signal-to-noise ratio and the limits of detection (LOD).

Original languageEnglish
Pages (from-to)473-478
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume395
Issue number2
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Microfluidics
Ultraviolet spectrophotometers
Spectrophotometers
Signal-To-Noise Ratio
Noise
Limit of Detection
Signal to noise ratio
Microscopes
Irradiation
Light

Keywords

  • Chip
  • External UV detection
  • Flow injection
  • Micro-spectrophotometer
  • Microfluidics/microfabrication
  • Poly(dimethylsiloxane)
  • UV/Vis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Application of external micro-spectrophotometric detection to improve sensitivity on microchips. / Gáspár, A.; Bácsi, István; Garcia, Erika F.; Braun, M.; Gomez, Frank A.

In: Analytical and Bioanalytical Chemistry, Vol. 395, No. 2, 09.2009, p. 473-478.

Research output: Contribution to journalArticle

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