Application of electric field stimulation for investigations of human placental blood vessels

Béla Endre Resch, R. Gáspár, G. Falkay

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

OBJECTIVE: To test electric field stimulation on human placental vessels. METHODS: The effects of electric field stimulation on placental vessels were examined in an isometric myograph. RESULTS: Electric field stimulation induced contractions in human placental blood vessels in vitro under isometric conditions when bubbling carbogen through the organ bath. After reaching half-maximal contractions, the vessel rings showed spontaneous relaxation. Pretreatment with verapamil (10-6 mol/L) or nickel (Ni2+) (2 mmol/L) inhibited the contractions to a magnitude of 63.81% ± 7.69% and 88.36% ± 12.17% (mean ± standard error of the mean), respectively. In calcium (Ca2+)-free medium after combined cyclopiazonic acid (10-5 mol/L) and Ni2+ treatment, it was not possible to elicit contractions with electric field stimulation. Bubbling through physiologic in utero hypoxic gases enhanced the contractile responses of the human placental vessel rings to electric field stimulation. The spontaneous relaxation of the veins was not altered, but those of the arteries were reduced to zero. Testing the same gases on mesenteric arteries of rats had an opposite effect concerning contractility. Sodium nitrite decreased the contractions of the placental vessel rings, but the efficacy was decreased by the in utero gases. CONCLUSION: Electric field stimulation has a direct, non-neurogenic contractile effect on isolated placental vessels, which mainly depends on the influx of extracellular Ca2+ and on a mechanism independent of intracellular Ca2+ concentration elevation. Physiologic hypoxia has a stimulatory effect on the contractility of human placental vessels, therefore in utero gases should be used instead of carbogen gas; and electric field stimulation is a suitable method for the investigation of the direct effects of pharmacologic agents on human placental vessels.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalObstetrics and Gynecology
Volume101
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

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Electric Stimulation
Blood Vessels
Gases
Sodium Nitrite
Mesenteric Arteries
Verapamil
Nickel
Baths
Veins
Arteries
Calcium

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Application of electric field stimulation for investigations of human placental blood vessels. / Resch, Béla Endre; Gáspár, R.; Falkay, G.

In: Obstetrics and Gynecology, Vol. 101, No. 2, 01.02.2003, p. 297-304.

Research output: Contribution to journalArticle

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N2 - OBJECTIVE: To test electric field stimulation on human placental vessels. METHODS: The effects of electric field stimulation on placental vessels were examined in an isometric myograph. RESULTS: Electric field stimulation induced contractions in human placental blood vessels in vitro under isometric conditions when bubbling carbogen through the organ bath. After reaching half-maximal contractions, the vessel rings showed spontaneous relaxation. Pretreatment with verapamil (10-6 mol/L) or nickel (Ni2+) (2 mmol/L) inhibited the contractions to a magnitude of 63.81% ± 7.69% and 88.36% ± 12.17% (mean ± standard error of the mean), respectively. In calcium (Ca2+)-free medium after combined cyclopiazonic acid (10-5 mol/L) and Ni2+ treatment, it was not possible to elicit contractions with electric field stimulation. Bubbling through physiologic in utero hypoxic gases enhanced the contractile responses of the human placental vessel rings to electric field stimulation. The spontaneous relaxation of the veins was not altered, but those of the arteries were reduced to zero. Testing the same gases on mesenteric arteries of rats had an opposite effect concerning contractility. Sodium nitrite decreased the contractions of the placental vessel rings, but the efficacy was decreased by the in utero gases. CONCLUSION: Electric field stimulation has a direct, non-neurogenic contractile effect on isolated placental vessels, which mainly depends on the influx of extracellular Ca2+ and on a mechanism independent of intracellular Ca2+ concentration elevation. Physiologic hypoxia has a stimulatory effect on the contractility of human placental vessels, therefore in utero gases should be used instead of carbogen gas; and electric field stimulation is a suitable method for the investigation of the direct effects of pharmacologic agents on human placental vessels.

AB - OBJECTIVE: To test electric field stimulation on human placental vessels. METHODS: The effects of electric field stimulation on placental vessels were examined in an isometric myograph. RESULTS: Electric field stimulation induced contractions in human placental blood vessels in vitro under isometric conditions when bubbling carbogen through the organ bath. After reaching half-maximal contractions, the vessel rings showed spontaneous relaxation. Pretreatment with verapamil (10-6 mol/L) or nickel (Ni2+) (2 mmol/L) inhibited the contractions to a magnitude of 63.81% ± 7.69% and 88.36% ± 12.17% (mean ± standard error of the mean), respectively. In calcium (Ca2+)-free medium after combined cyclopiazonic acid (10-5 mol/L) and Ni2+ treatment, it was not possible to elicit contractions with electric field stimulation. Bubbling through physiologic in utero hypoxic gases enhanced the contractile responses of the human placental vessel rings to electric field stimulation. The spontaneous relaxation of the veins was not altered, but those of the arteries were reduced to zero. Testing the same gases on mesenteric arteries of rats had an opposite effect concerning contractility. Sodium nitrite decreased the contractions of the placental vessel rings, but the efficacy was decreased by the in utero gases. CONCLUSION: Electric field stimulation has a direct, non-neurogenic contractile effect on isolated placental vessels, which mainly depends on the influx of extracellular Ca2+ and on a mechanism independent of intracellular Ca2+ concentration elevation. Physiologic hypoxia has a stimulatory effect on the contractility of human placental vessels, therefore in utero gases should be used instead of carbogen gas; and electric field stimulation is a suitable method for the investigation of the direct effects of pharmacologic agents on human placental vessels.

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