Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples

Carolina Cecilia Acebal, Hana Sklenářová, Jana Škrlíková, Ivana Šrámková, Vasil Andruch, I. Balogh, Petr Solich

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

An automated, simple and inexpensive double-valve sequential injection analysis (DV-SIA) spectrophotometic method with online liquid-liquid extraction, for the determination of thiocyanate has been developed. The method has been based on the formation of an ion associate between thiocyanate and Astra Phloxine in acidic medium, and the subsequent extraction with amylacetate. The absorbance of the extracted ion associate was measured at 550 nm. The calibration function was linear in the range 0.05-0.50 mmol L-1 and the regression equation was A = (1.887 ± 0.053) [SCN- mmol L-1] + (0.037 ± 0.014) with a correlation coefficient of 0.995. The precision of the proposed method was evaluated by the relative standard deviation (RSD) values at two concentration levels: 0.20 and 0.50 mmol L-1. The obtained results were 1.0 and 2.8%, respectively, for the intra-day precision, and 4.2 and 3.8%, respectively for the inter-day precision. The calculated detection limit was 0.02 mmol L-1. The developed method has been successfully applied for determining thiocyanate ions in human saliva samples.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalTalanta
Volume96
DOIs
Publication statusPublished - Jul 15 2012

Fingerprint

Saliva
Injections
Ions
Liquids
Liquid-Liquid Extraction
Calibration
Limit of Detection
thiocyanate

Keywords

  • Biological samples
  • DV-SIA
  • Extraction
  • Thiocyanate

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Acebal, C. C., Sklenářová, H., Škrlíková, J., Šrámková, I., Andruch, V., Balogh, I., & Solich, P. (2012). Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples. Talanta, 96, 107-112. https://doi.org/10.1016/j.talanta.2012.01.021

Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples. / Acebal, Carolina Cecilia; Sklenářová, Hana; Škrlíková, Jana; Šrámková, Ivana; Andruch, Vasil; Balogh, I.; Solich, Petr.

In: Talanta, Vol. 96, 15.07.2012, p. 107-112.

Research output: Contribution to journalArticle

Acebal, CC, Sklenářová, H, Škrlíková, J, Šrámková, I, Andruch, V, Balogh, I & Solich, P 2012, 'Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples', Talanta, vol. 96, pp. 107-112. https://doi.org/10.1016/j.talanta.2012.01.021
Acebal CC, Sklenářová H, Škrlíková J, Šrámková I, Andruch V, Balogh I et al. Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples. Talanta. 2012 Jul 15;96:107-112. https://doi.org/10.1016/j.talanta.2012.01.021
Acebal, Carolina Cecilia ; Sklenářová, Hana ; Škrlíková, Jana ; Šrámková, Ivana ; Andruch, Vasil ; Balogh, I. ; Solich, Petr. / Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples. In: Talanta. 2012 ; Vol. 96. pp. 107-112.
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