Application of different activating solutions to in vitro fertilization of crucian carp, Carassius carassius (L.), eggs

Daniel Zarski, A. Horváth, Gergely Bernáth, Katarzyna Palińska-Zarska, Sławomir Krejszeff, Tamás Müller, Dariusz Kucharczyk

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Within the study, the in vitro fertilization (ivF) in crucian carp, Carassius carassius (L.), with the use of three activating solutions (AS) [reverse osmosis water (RO), Woynarovich (WS) and Billard (BS) solutions] as well as the period of the capacity of eggs to be fertilized (up to 180 s post-egg-activation) in those solutions. Moreover, CASA analysis of sperm motility was conducted with each AS. In control groups (0 s), the highest (P <0.05) fertilization rate (93.2 %) was observed with WS. The application of RO and BS affected lower (P <0.05) embryo survival (63.5 and 0.05) between the groups were found considering the curvilinear velocity (VCL) 15 s post-sperm-activation. Between 30 and 135 s, the lowest (P <0.05) VCL after application of RO was noted. The highest VCL (P <0.05) up to 135 s was noted after application of BS and WS. All AS activated crucian carp sperm. The lowest fertilization rate was noted when BS was used, despite the high pMOT and VCL. It suggest that the AS which activates the sperm properly may not activate the eggs probably due to high osmolality of BS (262 mOsm kg-1). Because eggs retained the longest period of activity in WS, this AS is suggested for ivF in crucian carp.

Original languageEnglish
Pages (from-to)173-184
Number of pages12
JournalAquaculture International
Volume22
Issue number1
DOIs
Publication statusPublished - Feb 2014

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Carassius
Carassius carassius
in vitro fertilization
egg
reverse osmosis
sperm
fertilization (reproduction)
spermatozoa
sperm capacitation
water
motility
osmolality
sperm motility
embryo
embryo (animal)

Keywords

  • Carassius carassius
  • Crucian carp
  • Egg activation
  • Fertilization
  • Gamete management
  • Sperm activation
  • Sperm motility

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Aquatic Science

Cite this

Application of different activating solutions to in vitro fertilization of crucian carp, Carassius carassius (L.), eggs. / Zarski, Daniel; Horváth, A.; Bernáth, Gergely; Palińska-Zarska, Katarzyna; Krejszeff, Sławomir; Müller, Tamás; Kucharczyk, Dariusz.

In: Aquaculture International, Vol. 22, No. 1, 02.2014, p. 173-184.

Research output: Contribution to journalArticle

Zarski, Daniel ; Horváth, A. ; Bernáth, Gergely ; Palińska-Zarska, Katarzyna ; Krejszeff, Sławomir ; Müller, Tamás ; Kucharczyk, Dariusz. / Application of different activating solutions to in vitro fertilization of crucian carp, Carassius carassius (L.), eggs. In: Aquaculture International. 2014 ; Vol. 22, No. 1. pp. 173-184.
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