Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains

Orsolya Bouquet, Béla Kocsis, F. Kilár, Ildikó Kustos

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Chip-based fl ow cytometry is a rather new method that offers an easy, fast opportunity for examination of yeasts, such as Candida cells. In our study cell-chip technology was tested with ATCC Candida strains to determine their viability and susceptibility against antifungal agents, amphotericin B and fl uconazole. We found this technology to be suitable for the detection of Candida cells, for the differentiation between dead and living cells, and for the determination of amphotericin B and fl uconazole susceptibility of different Candida strains ( Bouquet et al., Mycoses 55:e90-e96, 2012).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages149-154
Number of pages6
Volume968
ISBN (Print)9781627032568
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume968
ISSN (Print)10643745

Fingerprint

Fluconazole
Amphotericin B
Candida
Flow Cytometry
Technology
Mycoses
Antifungal Agents
Cell Differentiation
Yeasts

Keywords

  • Agilent 2100 bioanalyzer
  • Amphotericin B
  • Candida
  • Flow cytometry
  • Fluconazole
  • Lab-on-a-chip
  • MIC

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bouquet, O., Kocsis, B., Kilár, F., & Kustos, I. (2013). Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains. In Methods in Molecular Biology (Vol. 968, pp. 149-154). (Methods in Molecular Biology; Vol. 968). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-257-5_11

Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains. / Bouquet, Orsolya; Kocsis, Béla; Kilár, F.; Kustos, Ildikó.

Methods in Molecular Biology. Vol. 968 Humana Press Inc., 2013. p. 149-154 (Methods in Molecular Biology; Vol. 968).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bouquet, O, Kocsis, B, Kilár, F & Kustos, I 2013, Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains. in Methods in Molecular Biology. vol. 968, Methods in Molecular Biology, vol. 968, Humana Press Inc., pp. 149-154. https://doi.org/10.1007/978-1-62703-257-5_11
Bouquet O, Kocsis B, Kilár F, Kustos I. Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains. In Methods in Molecular Biology. Vol. 968. Humana Press Inc. 2013. p. 149-154. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-257-5_11
Bouquet, Orsolya ; Kocsis, Béla ; Kilár, F. ; Kustos, Ildikó. / Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains. Methods in Molecular Biology. Vol. 968 Humana Press Inc., 2013. pp. 149-154 (Methods in Molecular Biology).
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