Application of capillary zone electrophoresis to the analysis and to a stability study of cephalosporins

A. Gáspár, Melinda Andrási, Szilvia Kardos

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The applicability of capillary zone electrophoresis (CZE) for the determination of cephalosporin antibiotics has been studied. In the case of the separation conditions optimised for fourteen cephalosporins, the precision of migration times was smaller than 1.3% RSD, and the values of the limit of detection ranged between 0.42 and 1.62 μg/ml. The proposed CZE method was applied to study the stability of cephalosporins in water at different temperatures (+25, +4 and -18°C). It was established that the degradation of most cephalosporins was not higher than 20% at room temperature within 4 h of dissolution of these antibiotics.

Original languageEnglish
Pages (from-to)239-246
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume775
Issue number2
DOIs
Publication statusPublished - Aug 5 2002

Fingerprint

Capillary Electrophoresis
Cephalosporins
Electrophoresis
Temperature
Limit of Detection
Dissolution
Anti-Bacterial Agents
Degradation
Water

Keywords

  • Cephalosporins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Application of capillary zone electrophoresis to the analysis and to a stability study of cephalosporins. / Gáspár, A.; Andrási, Melinda; Kardos, Szilvia.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 775, No. 2, 05.08.2002, p. 239-246.

Research output: Contribution to journalArticle

@article{8e4a902ea20c40b7ab752d574f7ff69a,
title = "Application of capillary zone electrophoresis to the analysis and to a stability study of cephalosporins",
abstract = "The applicability of capillary zone electrophoresis (CZE) for the determination of cephalosporin antibiotics has been studied. In the case of the separation conditions optimised for fourteen cephalosporins, the precision of migration times was smaller than 1.3{\%} RSD, and the values of the limit of detection ranged between 0.42 and 1.62 μg/ml. The proposed CZE method was applied to study the stability of cephalosporins in water at different temperatures (+25, +4 and -18°C). It was established that the degradation of most cephalosporins was not higher than 20{\%} at room temperature within 4 h of dissolution of these antibiotics.",
keywords = "Cephalosporins",
author = "A. G{\'a}sp{\'a}r and Melinda Andr{\'a}si and Szilvia Kardos",
year = "2002",
month = "8",
day = "5",
doi = "10.1016/S1570-0232(02)00311-2",
language = "English",
volume = "775",
pages = "239--246",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Application of capillary zone electrophoresis to the analysis and to a stability study of cephalosporins

AU - Gáspár, A.

AU - Andrási, Melinda

AU - Kardos, Szilvia

PY - 2002/8/5

Y1 - 2002/8/5

N2 - The applicability of capillary zone electrophoresis (CZE) for the determination of cephalosporin antibiotics has been studied. In the case of the separation conditions optimised for fourteen cephalosporins, the precision of migration times was smaller than 1.3% RSD, and the values of the limit of detection ranged between 0.42 and 1.62 μg/ml. The proposed CZE method was applied to study the stability of cephalosporins in water at different temperatures (+25, +4 and -18°C). It was established that the degradation of most cephalosporins was not higher than 20% at room temperature within 4 h of dissolution of these antibiotics.

AB - The applicability of capillary zone electrophoresis (CZE) for the determination of cephalosporin antibiotics has been studied. In the case of the separation conditions optimised for fourteen cephalosporins, the precision of migration times was smaller than 1.3% RSD, and the values of the limit of detection ranged between 0.42 and 1.62 μg/ml. The proposed CZE method was applied to study the stability of cephalosporins in water at different temperatures (+25, +4 and -18°C). It was established that the degradation of most cephalosporins was not higher than 20% at room temperature within 4 h of dissolution of these antibiotics.

KW - Cephalosporins

UR - http://www.scopus.com/inward/record.url?scp=0037025823&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037025823&partnerID=8YFLogxK

U2 - 10.1016/S1570-0232(02)00311-2

DO - 10.1016/S1570-0232(02)00311-2

M3 - Article

C2 - 12113991

AN - SCOPUS:0037025823

VL - 775

SP - 239

EP - 246

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 2

ER -