Application of biosensor with amperometric detection for determining ethanol

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Determination of ethanol content is one of the most important routinely performed tests in the field of wineries, breweries and liquor producers, and a sensor system has been developed for this purpose. The sensor was based on a thin-layer enzyme cell, in which alcohol oxidase (EC was immobilized on a protein membrane. The product of the enzyme reaction was determined by an amperometric technique. In the amperometric cell, platinum-Ag-AgCl-platinum electrodes were used. Having found the optimum conditions of pH, temperature and flow rate, the statistical parameters of the analysis were established. The linear range of determination was between 1 and 8% v/v of ethanol, and the detection limit was 0.1% v/v. When injecting standard solutions, the error was 0.14% v/v. The lifetime of the thin-layer cell was more than 3 weeks and more than 250 samples were analysed during this period. The ethanol content of several samples was determined with this sensor, and the results were compared with those of a manual method. The correlation coefficient in the range 4-7% v/v of ethanol was found to be 0.992. The results showed that ethanol can be determined, at the required sensitivity, by means of this biosensor.

Original languageEnglish
Pages (from-to)1843-1847
Number of pages5
JournalThe Analyst
Issue number8
Publication statusPublished - Dec 1 1994


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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