Application of an improved column characterisation system to evaluate the within and between batch variability

Erik Haghedooren, Agnes Kerner, B. Noszál, Jos Hoogmartens, Erwin Adams

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The selection of a reversed-phase liquid chromatographic column with suitable selectivity for a particular separation is difficult if the brand name of the column is not known. A project to develop a chromatographic test procedure to characterize reversed-phase liquid chromatography C18 columns was started earlier and resulted in a fast, simple, repeatable and reproducible test procedure using four column parameters. Here, this procedure is used to evaluate the diversity of columns originating from the same batch as well as from different batches. The determination of one of the parameters, the retention factor of 2,2′-dipyridyl, was improved and a simplified test procedure is proposed.

Original languageEnglish
Pages (from-to)634-639
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume44
Issue number3 SPEC. ISS.
DOIs
Publication statusPublished - Jul 27 2007

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Liquid chromatography
Liquids
Reverse-Phase Chromatography
Names

Keywords

  • C column
  • Reversed-phase liquid chromatography
  • Test parameters

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

Application of an improved column characterisation system to evaluate the within and between batch variability. / Haghedooren, Erik; Kerner, Agnes; Noszál, B.; Hoogmartens, Jos; Adams, Erwin.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 44, No. 3 SPEC. ISS., 27.07.2007, p. 634-639.

Research output: Contribution to journalArticle

Haghedooren, Erik ; Kerner, Agnes ; Noszál, B. ; Hoogmartens, Jos ; Adams, Erwin. / Application of an improved column characterisation system to evaluate the within and between batch variability. In: Journal of Pharmaceutical and Biomedical Analysis. 2007 ; Vol. 44, No. 3 SPEC. ISS. pp. 634-639.
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