Application of a rapid electrophoresis technique analysing the glutenin subunit composition of wheat genotypes

A. Kovács, M. Rakszegi, L. Láng, W. Ma, F. Békés, Z. Bedő

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The characterization of the old Hungarian varieties and landraces is an important part of Hungarian cereal research and breeding. Analysis of these germplasms with the most up-to-date methodologies results a broad scale of diversity of glutenin alleles, which proves their genetic heterogenicity. Exploitation of this attribute is an untapped possibility for developing modern varieties in our breeding programs. The previous research work revealed this diversity by SDS-PAGE analysis and MALDI-TOF technology. The powerful tool, the high throughput lab-on-a chip technique can facilitate the effectiveness of this function and decreases the cost of the analysis. This study demonstrates the application of this technique for analysing the old varieties. The allelic composition and their effects on bread making quality concluded by means of functional analysis.

Original languageEnglish
Pages (from-to)468-481
Number of pages14
JournalCereal Research Communications
Volume41
Issue number3
DOIs
Publication statusPublished - Sep 1 2013

Fingerprint

glutenins
Triticum
Breeding
electrophoresis
Electrophoresis
Genotype
wheat
genotype
Bread
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Research
Polyacrylamide Gel Electrophoresis
cost analysis
Alleles
breeding
Technology
Costs and Cost Analysis
breads
landraces
polyacrylamide gel electrophoresis

Keywords

  • glutenin
  • lab-on-a-chip
  • MALDI-TOF-MS
  • SE-HPLC
  • wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Physiology

Cite this

Application of a rapid electrophoresis technique analysing the glutenin subunit composition of wheat genotypes. / Kovács, A.; Rakszegi, M.; Láng, L.; Ma, W.; Békés, F.; Bedő, Z.

In: Cereal Research Communications, Vol. 41, No. 3, 01.09.2013, p. 468-481.

Research output: Contribution to journalArticle

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