Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell

Caroline I. Smith, Michele R.F. Siggel-King, James Ingham, Paul Harrison, David S. Martin, A. Varró, D. Mark Pritchard, Mark Surman, Steve Barrett, Peter Weightman

Research output: Contribution to journalArticle

Abstract

This work reports the first images obtained by combining an infrared aperture scanning near-field optical microscope (SNOM) with a quantum cascade laser (QCL). The future potential of this set-up is demonstrated by a preliminary study on an OE33 human oesophageal adenocarcinoma cell in which the cell is imaged at 1751 cm−1, 1651 cm−1, 1539 cm−1 and 1242 cm−1. In addition to the 1651 cm−1 image, three other images were acquired within the Amide I band (1689 cm−1, 1675 cm−1 and 1626 cm−1) chosen to correspond to secondary structures of proteins. The four images obtained within the Amide I band show distinct differences demonstrating the potential of this approach to reveal subtle changes in the chemical composition of a cell.

Original languageEnglish
Pages (from-to)5912-5917
Number of pages6
JournalAnalyst
Volume143
Issue number24
DOIs
Publication statusPublished - Dec 21 2018

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Smith, C. I., Siggel-King, M. R. F., Ingham, J., Harrison, P., Martin, D. S., Varró, A., Pritchard, D. M., Surman, M., Barrett, S., & Weightman, P. (2018). Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell. Analyst, 143(24), 5912-5917. https://doi.org/10.1039/c8an01183d