Application of a New Non-Porous Stationary Phase (Kovasil-H) for the Fast Separation of Peptides by HPLC

R. Ohmacht, I. Kiss

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Kovasil-H, a new 1.5 μm, non-porous, silica-based, reversed phase packing was investigated for the peptide mapping of Bovin Serum Albumin (BSA) and Cytochrome c tryptic digests and for the quality control of trypsin. Using short columns (33-66 mm) and high temperature, very short analysis times and high sensitivities were attained. No special modification of commonly used liquid chromatographs are necessary for the application of this method.

Original languageEnglish
Pages (from-to)595-598
Number of pages4
JournalChromatographia
Volume42
Issue number9-10
DOIs
Publication statusPublished - Jan 1 1996

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Keywords

  • Column liquid chromatography
  • Fast peptide separation
  • Non-porous, reversed phase packing
  • Peptide mapping

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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