Application of a New Non-Porous Stationary Phase (Kovasil-H) for the Fast Separation of Peptides by HPLC

R. Ohmacht, I. Kiss

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Kovasil-H, a new 1.5 μm, non-porous, silica-based, reversed phase packing was investigated for the peptide mapping of Bovin Serum Albumin (BSA) and Cytochrome c tryptic digests and for the quality control of trypsin. Using short columns (33-66 mm) and high temperature, very short analysis times and high sensitivities were attained. No special modification of commonly used liquid chromatographs are necessary for the application of this method.

Original languageEnglish
Pages (from-to)595-598
Number of pages4
JournalChromatographia
Volume42
Issue number9-10
Publication statusPublished - 1996

Fingerprint

Peptide Mapping
Cytochromes c
Serum Albumin
Silicon Dioxide
Quality Control
Trypsin
Quality control
High Pressure Liquid Chromatography
Peptides
Temperature
Liquids

Keywords

  • Column liquid chromatography
  • Fast peptide separation
  • Non-porous, reversed phase packing
  • Peptide mapping

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

Application of a New Non-Porous Stationary Phase (Kovasil-H) for the Fast Separation of Peptides by HPLC. / Ohmacht, R.; Kiss, I.

In: Chromatographia, Vol. 42, No. 9-10, 1996, p. 595-598.

Research output: Contribution to journalArticle

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