Application of a computer-directed automated microscope in mycobacteriology

Akos Somoskövi, Zs Györi, N. Czoboly, P. Magyar

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3 Citations (Scopus)

Abstract

Microscopy is currently the fastest, cheapest and most easily performed technique in mycobacteriology; it can be used in any laboratory. However, the sensitivity of microscopy is unsatisfactory and it is time-consuming. To eliminate these drawbacks, we have constructed a computer-directed automated microscope. To evaluate the equipment, we examined a total of 132 smears of sputum and 74 smears of liquid media. Manual microscopy was positive for 53 and negative for 79 sputum smears, while automated microscopy was positive for 55 and negative for 77 sputum smears. Both methods furnished 50 positive and 24 negative smears of liquid media. We conclude that the automated microscope is able to detect acid-fast bacteria, the examination procedure with the instrument is more rapid (1.8-3.5 min/slide) and it is always possible to follow the standard recommendations of microscopy.

Original languageEnglish
Pages (from-to)354-357
Number of pages4
JournalInternational Journal of Tuberculosis and Lung Disease
Volume3
Issue number4
Publication statusPublished - Apr 1 1999

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Keywords

  • Acid-fast microscopy
  • Automation
  • Mycobacteria

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

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