Applicability of forced-flow planar chromatographic methods for the separation of enantiomers on chiralplate®

S. Nyiredy, Karin Dallenbach-Toelke, Otto Sticher

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The separation of enantiomers using forced-flow planar chromatographic techniques such as overpressured layer chromatography and various rotation planar chromatographic methods such as ultra-microchamber rotation planar chromatography and microchamber rotation planar chromatography is reported for the first time. For circular and linear overpressured layer chromatography and ultra-microchamber rotation planar chromatography, the thin-layer chromatographic (TLC) mobile phase system from chromatography in an unsaturated chamber could be applied directly. In overpressured layer chromatography, a prerun had to be made before starting the separation in order to eliminate the disturbing zone. To achieve better migration of the α-front on the chiralplate® in ultra-microchamber rotation planar chromatography an area of 10-20 cm2 had to be wetted with a component of the mobile phase in which the substances did not migrate. The TLC mobile phase used in a saturated chamber exhibits similar properties when transferred to microchamber rotation planar chromatography. Employing forced-flow planar chromatographic techniques as many as 80 samples can be separated on a single plate. In all instances the separations were better and faster with the forced-flow methods than those achieved with TLC with the same mobile phase.

Original languageEnglish
Pages (from-to)241-252
Number of pages12
JournalJournal of Chromatography A
Volume450
Issue number2
DOIs
Publication statusPublished - 1988

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Enantiomers
Chromatography
Thin Layer Chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Applicability of forced-flow planar chromatographic methods for the separation of enantiomers on chiralplate®. / Nyiredy, S.; Dallenbach-Toelke, Karin; Sticher, Otto.

In: Journal of Chromatography A, Vol. 450, No. 2, 1988, p. 241-252.

Research output: Contribution to journalArticle

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abstract = "The separation of enantiomers using forced-flow planar chromatographic techniques such as overpressured layer chromatography and various rotation planar chromatographic methods such as ultra-microchamber rotation planar chromatography and microchamber rotation planar chromatography is reported for the first time. For circular and linear overpressured layer chromatography and ultra-microchamber rotation planar chromatography, the thin-layer chromatographic (TLC) mobile phase system from chromatography in an unsaturated chamber could be applied directly. In overpressured layer chromatography, a prerun had to be made before starting the separation in order to eliminate the disturbing zone. To achieve better migration of the α-front on the chiralplate{\circledR} in ultra-microchamber rotation planar chromatography an area of 10-20 cm2 had to be wetted with a component of the mobile phase in which the substances did not migrate. The TLC mobile phase used in a saturated chamber exhibits similar properties when transferred to microchamber rotation planar chromatography. Employing forced-flow planar chromatographic techniques as many as 80 samples can be separated on a single plate. In all instances the separations were better and faster with the forced-flow methods than those achieved with TLC with the same mobile phase.",
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