Applicability of empore™ TLC sheets for forced-flow planar chromatography. i. characterization of the silica sheets

L. Botz, S. Nyiredy, O. Sticher, E. Wehrli

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Empore™ TLC silica sheets - a new separation medium for planar chromatography - are prepared from silica which is entrapped in an inert matrix of polytetrafluoroethylene (PTFE) microfibrils. It was established earlier that the separation power is only about 60 % of that of conventional TLC. This was attributed to the very slow solvent migration velocity resulting from capillary action. In this paper the characteristics and applicability of Empore™ TLC silica sheets are reported for the various forced-flow planar chromatographic (FFPC) techniques, such as overpressured layer chromatography (OPLC) and rotation planar chromatography (RPC). The preparation of the sheets and the relationship between the solvent front migration distance and migration time are given. The influence of the linear flow rate on the theoretical plate height and the relationship between the resolution and the linear flow rate are determined. Also, the influence of the development mode and solvent system on the densitometric evaluation is discussed. The influence of external force (overpressure or centrifugal force) on the structure of Empore™ TLC silica sheets is demonstrated with the aid of scanning electron micrographs.

Original languageEnglish
Pages (from-to)2809-2828
Number of pages20
JournalJournal of Liquid Chromatography
Volume13
Issue number14
DOIs
Publication statusPublished - Aug 1 1990

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Chromatography
Silicon Dioxide
Flow rate
Capillary Action
Microfibrils
Polytetrafluoroethylene
Electrons
Scanning
Amberlite XAD-2 resin

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Applicability of empore™ TLC sheets for forced-flow planar chromatography. i. characterization of the silica sheets. / Botz, L.; Nyiredy, S.; Sticher, O.; Wehrli, E.

In: Journal of Liquid Chromatography, Vol. 13, No. 14, 01.08.1990, p. 2809-2828.

Research output: Contribution to journalArticle

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