Applicability of biochemical and genetic markers In the polymorphism analysis of maize lines

E. Nagy, T. Spitkó, L. C. Marton

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

guidelines elaborated by the Community Plant Variety Office (CPVO) of the European Union for the registration and patenting of varieties and hybrids of cultivated crops are based on the triple criteria of distinctiveness, uniformity and stability (DUS). For many species, however, morphological descriptions are not sufficient for the detection of differences between varieties. Techniques that allow varieties to be identified and distinguished precisel and reliably are essential not only for variety identification, but also to protect variety ownership rights. Biochemical and molecular genetic methods have now reached a level of developmen that makes them suitable for this purpose, and when these are combined with conventional field observations the breeding stock can be unequivocally identified and any existing genetic diver ity can be detected. The regular application of such analyses is a fundamental criterion in the case of maize, as more and more closely related hybrids are bein entered for testing.The aim of the present work was to examine ow isoenzyme patterns and PCR-based genetic markers could be used in polymorphism analysis, in order to obtain information on the genetic diversity of Hungari n breeding materials. The emphasis was on finding genetic markers characteristic of individual maize varieties, rather than of maize as a species.. copyright

Original languageEnglish
Pages (from-to)373-381
Number of pages9
JournalCereal Research Communications
Volume37
Issue number3
DOIs
Publication statusPublished - 2009

Keywords

  • Isoenzyme pattern
  • Maize
  • RAPD
  • SSR

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics

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