Appearance of Planktothrix rubescens bloom with [D-Asp3, Mdha7]MC-RR in gravel pit pond of a shallow lake-dominated area

G. Vasas, Oszkár Farkas, G. Borics, Tamás Felföldi, Gábor Sramkó, G. Batta, István Bácsi, Sándor Gonda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Blooms of toxic cyanobacteria are well-known phenomena in many regions of the world. Microcystin (MC), the most frequent cyanobacterial toxin, is produced by entirely different cyanobacteria, including unicellular, multicellular filamentous, heterocytic, and non-heterocytic bloom-forming species. Planktothrix is one of the most important MC-producing genera in temperate lakes. The reddish color of cyanobacterial blooms viewed in a gravel pit pond with the appearance of a dense 3 cm thick layer (biovolume: 28.4 mm3 L-1) was an unexpected observation in the shallow lake-dominated alluvial region of the Carpathian Basin. [D-Asp3, Mdha7]MC-RR was identified from the blooms sample by MALDI-TOF and NMR. Concentrations of [D-Asp3, Mdha7]MC-RR were measured by capillary electrophoresis to compare the microcystin content of the field samples and the isolated, laboratory-maintained P. rubescens strain. In analyzing the MC gene cluster of the isolated P. rubescens strain, a deletion in the spacer region between mcyE and mcyG and an insertion were located in the spacer region between mcyT and mcyD. The insertion elements were sequenced and partly identified. Although some invasive tropical cyanobacterial species have been given a great deal of attention in many recent studies, our results draw attention to the spread of the alpine organism P. rubescens as a MC-producing, bloom-forming species.

Original languageEnglish
Pages (from-to)2434-2455
Number of pages22
JournalToxins
Volume5
Issue number12
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Ponds
Gravel
Lakes
Cyanobacteria
Capillary electrophoresis
Poisons
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Capillary Electrophoresis
Multigene Family
Catchments
Color
Genes
Nuclear magnetic resonance
Observation
microcystin RR
microcystin

Keywords

  • Cyanobacteria
  • MALDI-TOF
  • Microcystins
  • Planktothrix
  • Waterbloom

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Appearance of Planktothrix rubescens bloom with [D-Asp3, Mdha7]MC-RR in gravel pit pond of a shallow lake-dominated area. / Vasas, G.; Farkas, Oszkár; Borics, G.; Felföldi, Tamás; Sramkó, Gábor; Batta, G.; Bácsi, István; Gonda, Sándor.

In: Toxins, Vol. 5, No. 12, 12.2013, p. 2434-2455.

Research output: Contribution to journalArticle

Vasas, G. ; Farkas, Oszkár ; Borics, G. ; Felföldi, Tamás ; Sramkó, Gábor ; Batta, G. ; Bácsi, István ; Gonda, Sándor. / Appearance of Planktothrix rubescens bloom with [D-Asp3, Mdha7]MC-RR in gravel pit pond of a shallow lake-dominated area. In: Toxins. 2013 ; Vol. 5, No. 12. pp. 2434-2455.
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