Appearance of deteriorated neurons on regionally different time tables in rat brain thin slices maintained in physiological condition

Atsuo Fukuda, A. Czurkó, Hideki Hida, Kanji Muramatsu, L. Lénárd, Hitoo Nishino

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Brain slices are widelly used for electrophysiological experiments. However, the time table of the chronological cell deterioration and its regional difference in slices are unknown. The argyrophil III staining method can demonstrate deteriorated ('collapsed') neurons specifically. Therefore we studied the appearance of the 'collapsed' neurons with time in coronal brain 'thin' slices maintained in vitro to evaluate possible regional differences in vulnerability. In the hippocampus, CAI pyramidal, dentate gyrus granule, and non-pyramidal cells in any subfield became argyrophilic most easily, as did layer V-VI pyramidal neurons in the neocortex. These results suggest that some subclasses of neurons in brain slices degrade earlier than others even when maintained in physiological condition.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalNeuroscience Letters
Volume184
Issue number1
DOIs
Publication statusPublished - Jan 16 1995

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Neurons
Brain
Pyramidal Cells
Neocortex
Dentate Gyrus
Hippocampus
Staining and Labeling
In Vitro Techniques

Keywords

  • Argyrophil III method
  • Brain slice
  • Cell death
  • Collapsed neuron
  • Cytoskeletal damage
  • Hippocampus
  • Neocortex
  • Neuronal deterioration

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Appearance of deteriorated neurons on regionally different time tables in rat brain thin slices maintained in physiological condition. / Fukuda, Atsuo; Czurkó, A.; Hida, Hideki; Muramatsu, Kanji; Lénárd, L.; Nishino, Hitoo.

In: Neuroscience Letters, Vol. 184, No. 1, 16.01.1995, p. 13-16.

Research output: Contribution to journalArticle

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