Appearance and phenotype of murine follicular dendritic cells expressing VCAM-1

Péter Balogh, Yüksel Aydar, John G. Tew, Andras K. Szakal

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The architecture of lymphoid follicles is determined by a series of interactions between lymphoid and follicular stromal cells. A cardinal population in the non-lymphoid compartment is the follicular dendritic cell (FDC), whose communication with resting and activated B cells involves various adhesive interactions. The FDC phenotype variably includes the display of vascular cell adhesion molecule (VCAM-1). In this report we investigated the appearance and follicular tissue distribution of VCAM-1 in murine peripheral lymphoid tissues, and compared VCAM-1 with other FDC markers using immunohistochemistry. Correlating the appearance of VCAM-1 with other murine FDC-associated markers (CR1.2 [complement receptor 1.2 or CD35/21] and FDC-M1) revealed that the display of VCAM-1 is restricted to a subset of CR1.2-positive FDCs. We found that the expression of VCAM-1 antigen in the spleen or peripheral lymph nodes on FDCs requires antigenic stimulus, and that it coincides with germinal center formation. The VCAM-1 expression is associated with the appearance of mucosal addressin cell adhesion molecule (MAdCAM-1), with some slight differences in occurrence. The appearance of VCAM-1 and MAdCAM-1 antigens on FDCs may serve as indicators of FDC activation.

Original languageEnglish
Pages (from-to)160-168
Number of pages9
JournalAnatomical Record
Volume268
Issue number2
DOIs
Publication statusPublished - Oct 1 2002

Keywords

  • Follicular dendritic cells
  • Germinal center
  • Immunohistochemistry
  • VCAM-1

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

Fingerprint Dive into the research topics of 'Appearance and phenotype of murine follicular dendritic cells expressing VCAM-1'. Together they form a unique fingerprint.

  • Cite this