Apoptotic neurons induce proliferative responses of progenitor cells in the postnatal neocortex

Volodymyr Petrenko, Jevgenia Mihhailova, Patrick Salmon, J. Kiss

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Apoptotic cell death is the leading cause of neuronal loss after neonatal brain injury. Little is known about the intrinsic capacity of the immature cerebral cortex for replacing dead cells. Here we test the hypothesis that neuronal apoptosis is able to trigger compensatory proliferation in surrounding cells. In order to establish a "pure" apoptotic cell death model and to avoid the confounding effects of broken blood-brain barrier and inflammatory reactions, we used a diphtheria toxin (DT) and diphtheria toxin receptor (DTR) system to induce ablation of layer IV neurons in the rodent somatosensory cortex during the early postnatal period. We found that DT-triggered apoptosis is a slowly progressing event lasting about for 7. days. While dying cells expressed the morphological features of apoptosis, we could not detect immunoreactivity for activated caspase-3 in these cells. Microglia activation and proliferation represented the earliest cellular responses to apoptotic cell death. In addition, we found that induced apoptosis triggered a massive proliferation of undifferentiated progenitor cell pool including Sox2 as well as NG2 cells. The default differentiation pattern of proliferating progenitors appears to be the glial phenotype; we could not find evidence for newly generated neurons in response to apoptotic neuronal death. These results suggest that mitotically active progenitor populations are intrinsically capable to contribute to the repair process of injured cortical tissue and may represent a potential target for neuronal replacement strategies.

Original languageEnglish
Article number12102
Pages (from-to)126-137
Number of pages12
JournalExperimental Neurology
Volume273
DOIs
Publication statusPublished - Nov 1 2015

Fingerprint

Neocortex
Stem Cells
Neurons
Apoptosis
Diphtheria Toxin
Cell Death
Somatosensory Cortex
Microglia
Blood-Brain Barrier
Neuroglia
Caspase 3
Cerebral Cortex
Brain Injuries
Rodentia
Phenotype
Population

Keywords

  • Apoptosis
  • Developing brain
  • Diphtheria toxin
  • Neural progenitors
  • Neurogenesis

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Apoptotic neurons induce proliferative responses of progenitor cells in the postnatal neocortex. / Petrenko, Volodymyr; Mihhailova, Jevgenia; Salmon, Patrick; Kiss, J.

In: Experimental Neurology, Vol. 273, 12102, 01.11.2015, p. 126-137.

Research output: Contribution to journalArticle

Petrenko, Volodymyr ; Mihhailova, Jevgenia ; Salmon, Patrick ; Kiss, J. / Apoptotic neurons induce proliferative responses of progenitor cells in the postnatal neocortex. In: Experimental Neurology. 2015 ; Vol. 273. pp. 126-137.
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