Apoptotic cell death induced by inhibitors of energy conservation Bcl-2 inhibits apoptosis downsbream of a fall of ATP level

Attila Marton, Rudolf Mihalik, András Bratincsák, Vilmos Adleff, István Peták, Miklós Végh, Pál I. Bauer, Péter Krajcsi

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Energy charge controls intermediary metabolism and cellular regulation. Here we show that inhibition of energy conservation at the level of glucose uptake, glycolysis, citric acid cycle, and oxidative phosphorylation induces cell death, leading to fragmentation of DNA into an oligonucleosomal ladder and morphological changes typical for apoptosis. Bcl-2, the prototype of oncogenes that suppress cell death, efficiently inhibits apoptosis induced by metabolic inhibitors. Bcl does not antagonize the inhibitory potential of mitochondrial inhibitors, and cannot prevent or delay the decrease of the cellular ATP level subsequent to metabolic inhibition. Thus, we propose that Bcl blocks apoptosis at a point downstream of the collapse of the cellular-energy homeostasis.

Original languageEnglish
Pages (from-to)467-475
Number of pages9
JournalEuropean Journal of Biochemistry
Volume250
Issue number2
DOIs
Publication statusPublished - Jan 1 1997

Keywords

  • ATP
  • Apoptosis
  • Bcl-2
  • Energy conservation
  • Metabolic regulation

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Apoptotic cell death induced by inhibitors of energy conservation Bcl-2 inhibits apoptosis downsbream of a fall of ATP level'. Together they form a unique fingerprint.

  • Cite this