Apoptotic and non-apoptotic modes of programmed cell death in MCF-7 human breast carcinoma cells

Farhana Amin, Ivor D. Bowen, Zsolt Szegedi, R. Mihalik, B. Szende

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Apoptosis is a specific mode of programmed cell death (PCD), recognized by characteristic morphological and molecular changes. Here we present evidence for a non-apoptotic type of PCD in human MCF-7 breast carcinoma cells. We used TNF-α and tyrphostin AG213 to induce apoptotic and non-apoptotic cell death respectively in vitro. Microscopic and immunohistochemical studies, together with DNA analysis and flow cytometric analysis of p53 and bcl-2 oncogene expression, revealed some novel characteristics of non-apoptotic cell death. We show here for the first time some of the biochemical features of an experimentally induced non-apoptotic PCD and emphasize the distinct biochemical events leading to apoptotic and non-apoptotic PCD.

Original languageEnglish
Pages (from-to)253-260
Number of pages8
JournalCell Biology International
Volume24
Issue number4
DOIs
Publication statusPublished - 2000

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Cell Death
Breast Neoplasms
Oncogenes
Apoptosis
DNA

Keywords

  • Apoptosis
  • MFC-7 breast carcinoma
  • Non-apoptotic programmed cell death
  • TNF-α
  • Tyrphostin AG213

ASJC Scopus subject areas

  • Cell Biology

Cite this

Apoptotic and non-apoptotic modes of programmed cell death in MCF-7 human breast carcinoma cells. / Amin, Farhana; Bowen, Ivor D.; Szegedi, Zsolt; Mihalik, R.; Szende, B.

In: Cell Biology International, Vol. 24, No. 4, 2000, p. 253-260.

Research output: Contribution to journalArticle

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