Apoptotic agents inducing genotoxicity-specific chromatin changes

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To visualize characteristic chromatin distortions we have distinguished first among regularly occurring intermediates of chromatin structures in mammalian (Indian muntjac, CHO, murine preB, rat liver, rat myeloid leukemia, K562 human erythroid leukemia) and Drosophila nuclei. Fluorescence microscopy of chromatin structures isolated from nuclei of reversibly permeable cells revealed a common pathway of chromatin condensation in mammalian cells. Different intermediates in mammalian and Drosophila cells indicate alternative mechanisms of chromosome condensation. Genotoxic agents such as irradiation (alpha, gamma, UV-B) and heavy metals (Cd, Pb, Ni, Hg, Ag) caused alterations in chromatin structures leading to apoptosis. Injury-specific chromatin changes manifested at significantly lower concentrations than non-specific signs of cellular toxicity, suggesting that preapoptotic events are useful indicators of genotoxicity.

Original languageEnglish
Pages (from-to)1301-1316
Number of pages16
JournalApoptosis
Volume19
Issue number9
DOIs
Publication statusPublished - 2014

Fingerprint

Chromatin
Drosophila
Rats
Condensation
Muntjacs
Myeloid Leukemia
Fluorescence microscopy
Chromosomes
Heavy Metals
Fluorescence Microscopy
Liver
Toxicity
Leukemia
Cells
Irradiation
Apoptosis
Wounds and Injuries

Keywords

  • Apoptotic chromatin changes
  • Chromosome condensation
  • Genotoxic agents
  • Indicators of apoptosis
  • Interphase chromatin

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biochemistry, medical
  • Cancer Research
  • Pharmaceutical Science
  • Pharmacology
  • Medicine(all)

Cite this

Apoptotic agents inducing genotoxicity-specific chromatin changes. / Bánfalvi, G.

In: Apoptosis, Vol. 19, No. 9, 2014, p. 1301-1316.

Research output: Contribution to journalArticle

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