Apoptosis-linked in vivo regulation of the tissue transglutaminase gene promoter

É Szegezdi, Z. Szondy, L. Nagy, Z. Nemes, R. R. Friis, P. J.A. Davies, L. Fésüs

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Tissue transglutaminase (tTG) is upregulated in various cells undergoing apoptosis. To investigate the transcriptional regulation of tTG a mouse strain carrying a β-galactosidase reporter gene under the control of a 3.8 kilobase fragment of the tTG promoter was characterised. The transgene construct was shown to be expressed in the apoptotic regions of the mouse embryo. Here we report that the regulation of the transgene is also apoptosis-linked in adult animals. The transgene is induced in endocrine apoptosis involving mammary gland involution and corpus luteum regression. Induction of the reporter gene is detectable during in vivo but not in vitro apoptosis of thymocytes induced by the glucocorticoid receptor, the nur77, p53 and the retinoid receptorγ mediated pathways. Additionally, the lacZ expression mimics the activation of the endogenous promoter in tissues characterised by high apoptotic turnover. These results suggest that the apoptosis-specific transcriptional regulation of tTG is mediated through elements of a 3.8 kb promoter and may require cosignals available only in tissue environment.

Original languageEnglish
Pages (from-to)1225-1233
Number of pages9
JournalCell Death and Differentiation
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 1 2000

Keywords

  • Apoptosis
  • Transgene mouse
  • Transglutaminase promoter

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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