Anxiolytic effect of neurotensin microinjection into the ventral pallidum

Tamás Ollmann, László Péczely, Kristóf László, Anita Kovács, Rita Gálosi, Erika Kertes, Veronika Kállai, Olga Zagorácz, Zoltán Karádi, L. Lénárd

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Neurotensin (NT) acts as a neurotransmitter and neuromodulator in the central nervous system. NT is involved in reward and memory processes, drug addiction and also in the regulation of anxiety. The ventral pallidum (VP) receives neurotensinergic innervation from the ventral striatopallidal pathway originating from the nucleus accumbens. Positive reinforcing effects of NT in the VP had been shown recently, however the possible effects of NT on anxiety have not been examined yet. In our present experiments, the effects of NT on anxiety were investigated in the VP. In male Wistar rats bilateral microinjections of 100. ng or 250. ng NT were delivered in the volume of 0.4. μl into the VP, and elevated plus maze (EPM) test was performed. In another groups of animals, 35. ng NT-receptor 1 (NTR1) antagonist SR 48,692 was applied by itself, or microinjected 15. min before 100. ng NT treatment. Open field test (OPF) was also conducted. The 100. ng dose of NT had anxiolytic effect, but the 250. ng NT did not influence anxiety. The antagonist pretreatment inhibited the effect of NT, while the antagonist itself had no effect. In the OPF test there was no difference among the groups. Our present results show that microinjection of NT into the VP induces anxiolytic effect, which is specific to the NTR1 receptors because it can be eliminated by a specific NTR1 antagonist. It is also substantiated that neither the NT, nor the NTR1 antagonist in the VP influences locomotor activity.

Original languageEnglish
Article number9760
Pages (from-to)208-214
Number of pages7
JournalBehavioural Brain Research
Volume294
DOIs
Publication statusPublished - Nov 1 2015

Fingerprint

Neurotensin
Anti-Anxiety Agents
Microinjections
Anxiety
Neurotransmitter Agents
Basal Forebrain
Nucleus Accumbens
Locomotion
Reward
Substance-Related Disorders
Wistar Rats
Central Nervous System

Keywords

  • Anxiety
  • Elevated plus maze test
  • Neurotensin
  • NTR1 antagonist
  • Open field test
  • Ventral pallidum

ASJC Scopus subject areas

  • Behavioral Neuroscience

Cite this

Ollmann, T., Péczely, L., László, K., Kovács, A., Gálosi, R., Kertes, E., ... Lénárd, L. (2015). Anxiolytic effect of neurotensin microinjection into the ventral pallidum. Behavioural Brain Research, 294, 208-214. [9760]. https://doi.org/10.1016/j.bbr.2015.08.010

Anxiolytic effect of neurotensin microinjection into the ventral pallidum. / Ollmann, Tamás; Péczely, László; László, Kristóf; Kovács, Anita; Gálosi, Rita; Kertes, Erika; Kállai, Veronika; Zagorácz, Olga; Karádi, Zoltán; Lénárd, L.

In: Behavioural Brain Research, Vol. 294, 9760, 01.11.2015, p. 208-214.

Research output: Contribution to journalArticle

Ollmann, T, Péczely, L, László, K, Kovács, A, Gálosi, R, Kertes, E, Kállai, V, Zagorácz, O, Karádi, Z & Lénárd, L 2015, 'Anxiolytic effect of neurotensin microinjection into the ventral pallidum', Behavioural Brain Research, vol. 294, 9760, pp. 208-214. https://doi.org/10.1016/j.bbr.2015.08.010
Ollmann T, Péczely L, László K, Kovács A, Gálosi R, Kertes E et al. Anxiolytic effect of neurotensin microinjection into the ventral pallidum. Behavioural Brain Research. 2015 Nov 1;294:208-214. 9760. https://doi.org/10.1016/j.bbr.2015.08.010
Ollmann, Tamás ; Péczely, László ; László, Kristóf ; Kovács, Anita ; Gálosi, Rita ; Kertes, Erika ; Kállai, Veronika ; Zagorácz, Olga ; Karádi, Zoltán ; Lénárd, L. / Anxiolytic effect of neurotensin microinjection into the ventral pallidum. In: Behavioural Brain Research. 2015 ; Vol. 294. pp. 208-214.
@article{052a7042f351459b9805985c6b5e08d5,
title = "Anxiolytic effect of neurotensin microinjection into the ventral pallidum",
abstract = "Neurotensin (NT) acts as a neurotransmitter and neuromodulator in the central nervous system. NT is involved in reward and memory processes, drug addiction and also in the regulation of anxiety. The ventral pallidum (VP) receives neurotensinergic innervation from the ventral striatopallidal pathway originating from the nucleus accumbens. Positive reinforcing effects of NT in the VP had been shown recently, however the possible effects of NT on anxiety have not been examined yet. In our present experiments, the effects of NT on anxiety were investigated in the VP. In male Wistar rats bilateral microinjections of 100. ng or 250. ng NT were delivered in the volume of 0.4. μl into the VP, and elevated plus maze (EPM) test was performed. In another groups of animals, 35. ng NT-receptor 1 (NTR1) antagonist SR 48,692 was applied by itself, or microinjected 15. min before 100. ng NT treatment. Open field test (OPF) was also conducted. The 100. ng dose of NT had anxiolytic effect, but the 250. ng NT did not influence anxiety. The antagonist pretreatment inhibited the effect of NT, while the antagonist itself had no effect. In the OPF test there was no difference among the groups. Our present results show that microinjection of NT into the VP induces anxiolytic effect, which is specific to the NTR1 receptors because it can be eliminated by a specific NTR1 antagonist. It is also substantiated that neither the NT, nor the NTR1 antagonist in the VP influences locomotor activity.",
keywords = "Anxiety, Elevated plus maze test, Neurotensin, NTR1 antagonist, Open field test, Ventral pallidum",
author = "Tam{\'a}s Ollmann and L{\'a}szl{\'o} P{\'e}czely and Krist{\'o}f L{\'a}szl{\'o} and Anita Kov{\'a}cs and Rita G{\'a}losi and Erika Kertes and Veronika K{\'a}llai and Olga Zagor{\'a}cz and Zolt{\'a}n Kar{\'a}di and L. L{\'e}n{\'a}rd",
year = "2015",
month = "11",
day = "1",
doi = "10.1016/j.bbr.2015.08.010",
language = "English",
volume = "294",
pages = "208--214",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",

}

TY - JOUR

T1 - Anxiolytic effect of neurotensin microinjection into the ventral pallidum

AU - Ollmann, Tamás

AU - Péczely, László

AU - László, Kristóf

AU - Kovács, Anita

AU - Gálosi, Rita

AU - Kertes, Erika

AU - Kállai, Veronika

AU - Zagorácz, Olga

AU - Karádi, Zoltán

AU - Lénárd, L.

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Neurotensin (NT) acts as a neurotransmitter and neuromodulator in the central nervous system. NT is involved in reward and memory processes, drug addiction and also in the regulation of anxiety. The ventral pallidum (VP) receives neurotensinergic innervation from the ventral striatopallidal pathway originating from the nucleus accumbens. Positive reinforcing effects of NT in the VP had been shown recently, however the possible effects of NT on anxiety have not been examined yet. In our present experiments, the effects of NT on anxiety were investigated in the VP. In male Wistar rats bilateral microinjections of 100. ng or 250. ng NT were delivered in the volume of 0.4. μl into the VP, and elevated plus maze (EPM) test was performed. In another groups of animals, 35. ng NT-receptor 1 (NTR1) antagonist SR 48,692 was applied by itself, or microinjected 15. min before 100. ng NT treatment. Open field test (OPF) was also conducted. The 100. ng dose of NT had anxiolytic effect, but the 250. ng NT did not influence anxiety. The antagonist pretreatment inhibited the effect of NT, while the antagonist itself had no effect. In the OPF test there was no difference among the groups. Our present results show that microinjection of NT into the VP induces anxiolytic effect, which is specific to the NTR1 receptors because it can be eliminated by a specific NTR1 antagonist. It is also substantiated that neither the NT, nor the NTR1 antagonist in the VP influences locomotor activity.

AB - Neurotensin (NT) acts as a neurotransmitter and neuromodulator in the central nervous system. NT is involved in reward and memory processes, drug addiction and also in the regulation of anxiety. The ventral pallidum (VP) receives neurotensinergic innervation from the ventral striatopallidal pathway originating from the nucleus accumbens. Positive reinforcing effects of NT in the VP had been shown recently, however the possible effects of NT on anxiety have not been examined yet. In our present experiments, the effects of NT on anxiety were investigated in the VP. In male Wistar rats bilateral microinjections of 100. ng or 250. ng NT were delivered in the volume of 0.4. μl into the VP, and elevated plus maze (EPM) test was performed. In another groups of animals, 35. ng NT-receptor 1 (NTR1) antagonist SR 48,692 was applied by itself, or microinjected 15. min before 100. ng NT treatment. Open field test (OPF) was also conducted. The 100. ng dose of NT had anxiolytic effect, but the 250. ng NT did not influence anxiety. The antagonist pretreatment inhibited the effect of NT, while the antagonist itself had no effect. In the OPF test there was no difference among the groups. Our present results show that microinjection of NT into the VP induces anxiolytic effect, which is specific to the NTR1 receptors because it can be eliminated by a specific NTR1 antagonist. It is also substantiated that neither the NT, nor the NTR1 antagonist in the VP influences locomotor activity.

KW - Anxiety

KW - Elevated plus maze test

KW - Neurotensin

KW - NTR1 antagonist

KW - Open field test

KW - Ventral pallidum

UR - http://www.scopus.com/inward/record.url?scp=84940892772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940892772&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2015.08.010

DO - 10.1016/j.bbr.2015.08.010

M3 - Article

C2 - 26296669

AN - SCOPUS:84940892772

VL - 294

SP - 208

EP - 214

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

M1 - 9760

ER -