Anxiogenic role of vasopressin during the early postnatal period: Maternal separation-induced ultrasound vocalization in vasopressin-deficient Brattleboro rats

János Varga, Anna Fodor, Barbara Klausz, D. Zelena

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Both animal and human studies suggest that in adulthood, plasma vasopressin level correlates well with anxiety. Little is known about the mood regulation during the perinatal period. Here, we aim to investigate the influence of vasopressin on anxiety during the early postnatal age. As a sign of distress, rat pups emit ultrasonic vocalizations (USVs) when they are separated from their mother. This USV was detected in 7- to 8-day-old vasopressin-deficient Brattleboro pups, and they were compared to their heterozygote littermates and wild-type pups. The results were confirmed by V1b antagonist treatment (SSR149415 10 mg/kg IP 30 min before test) in wild-types. Chlordiazepoxide (3 mg/kg IP 30 min before test) - an anxiolytic - was used to test the interaction with the GABAergic system. At the end of the test, stress-hormone levels were measured by radioimmunoassay. Vasopressin-deficient pups vocalized substantially less than non-deficient counterparts. Treatment with V1b antagonist resulted in similar effect. Chlordiazepoxide reduced the frequency and duration of the vocalization only in wild-types. Reduced vocalization was accompanied by smaller adrenocorticotropin levels but the level of corticosterone was variable. Our results indicate that the anxiolytic effect of vasopressin deficiency (both genetic and pharmacological) exists already during the early postnatal age. Vasopressin interacts with the GABAergic system. As mood regulation does not go parallel with glucocorticoid levels, we suggest that vasopressin might have a direct effect on special brain areas.

Original languageEnglish
Pages (from-to)2409-2418
Number of pages10
JournalAmino Acids
Volume47
Issue number11
DOIs
Publication statusPublished - Jul 2 2015

Fingerprint

Brattleboro Rats
Vasopressins
Rats
Ultrasonics
Mothers
Chlordiazepoxide
Anti-Anxiety Agents
Anxiety
Neurogenic Diabetes Insipidus
Heterozygote
Corticosterone
Exercise Test
Adrenocorticotropic Hormone
Glucocorticoids
Radioimmunoassay
Brain
Hormones
Pharmacology
Animals
Plasmas

Keywords

  • Anxiety
  • Chlordiazepoxide
  • GABA
  • Postnatal period
  • SSR149415
  • Ultrasound vocalization
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Anxiogenic role of vasopressin during the early postnatal period : Maternal separation-induced ultrasound vocalization in vasopressin-deficient Brattleboro rats. / Varga, János; Fodor, Anna; Klausz, Barbara; Zelena, D.

In: Amino Acids, Vol. 47, No. 11, 02.07.2015, p. 2409-2418.

Research output: Contribution to journalArticle

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