Antiviral activities of extracts of Euphorbia hirta L. against HIV-1, HIV-2 and SIV mac251

Ágnes Gyuris, László Szlávik, J. Mináróvits, A. Vasas, J. Molnár, J. Hohmann

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The antiretroviral activities of extracts of Euphorbia hirta were investigated in vitro on the MT4 human T lymphocyte cell line. The cytotoxicities of the extracts were tested by means of the MTT cell proliferation assay, and then the direct effects of the aqueous extract on HTV-1, HIV-2 and SIV mac251 reverse transcriptase (RT) activity were determined. A dose-dependent inhibition of RT activity was observed for all three viruses. The HIV-1 inhibitory potency of E. hirta was studied further, and the activities of the aqueous and 50% methanolic extracts were compared. The 50% methanolic extract was found to exert a higher antiretroviral effect than that of the aqueous extract. The 50% MeOH extract was subjected to liquid-liquid partition with dichloromethane, ethyl acetate and water. Only the remaining aqueous phase exhibited significant antiviral activity; all the lipophilic extracts appeared to be inactive. After removal of the tannins from the aqueous extract, the viral replication inhibitory effect was markedly decreased, and it was therefore concluded that tannins are most probably responsible for the high antiretroviral activity.

Original languageEnglish
Pages (from-to)429-432
Number of pages4
JournalIn Vivo
Volume23
Issue number3
Publication statusPublished - May 2009

Fingerprint

Euphorbia
HIV-2
Tannins
RNA-Directed DNA Polymerase
Antiviral Agents
HIV-1
T-cells
Methylene Chloride
Cell proliferation
Liquids
Cytotoxicity
Viruses
Assays
Cells
Cell Proliferation
T-Lymphocytes
Cell Line
Water
ethyl acetate
In Vitro Techniques

Keywords

  • Antiviral activity
  • Euphorbia hirta
  • Euphorbiaceae
  • HIV-2
  • HTV-1
  • SIV

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Antiviral activities of extracts of Euphorbia hirta L. against HIV-1, HIV-2 and SIV mac251 . / Gyuris, Ágnes; Szlávik, László; Mináróvits, J.; Vasas, A.; Molnár, J.; Hohmann, J.

In: In Vivo, Vol. 23, No. 3, 05.2009, p. 429-432.

Research output: Contribution to journalArticle

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