Antitumour properties of acridone alkaloids on a murine lymphoma cell line

B. Réthy, J. Hohmann, Renáta Minorics, András Varga, I. Ocsovszki, J. Molnár, Kata Juhász, G. Falkay, I. Zupkó

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The aim of the present study was to investigate the anticancer properties of a set of furanoacridone alkaloids, arborinine and evoxanthine, including the inhibitory effect of P-glycoprotein (Pgp) and the apoptosis-inducing capacity. The tested alkaloids were evaluated for multidrug resistance (MDR)-reversing activity on human Pgp-transfected L5178 mouse lymphoma cells, using the rhodamine-123 (Rh-123) assay. The antiproliferative effects of natural compounds and their interactions with doxorubicin were determined in MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. Apoptosis-inducing activity was additionally measured by means of dual annexin V and propidium iodide staining. RT-PCR was used to test the expression of Pgp mRNA after acridone treatment. All of the acridones investigated increased the accumulation of Rh-123. Gravacridonetriol and gravacridonediol monomethyl ether increased the antiproliferative effect of doxorubicin on resistant L5I78 cells. Treatment with these agents resulted in a decrease in Pgp mRNA levels. Naturally occurring acridone alkaloids exhibit a beneficial combination of anticancer effects and, accordingly, the acridone skeleton can be considered useful in the design of novel antiproliferative agents.

Original languageEnglish
Pages (from-to)2737-2743
Number of pages7
JournalAnticancer Research
Volume28
Issue number5 A
Publication statusPublished - Sep 2008

Fingerprint

P-Glycoprotein
Alkaloids
Lymphoma
Rhodamine 123
Cell Line
Leukemia L5178
Doxorubicin
Acridones
Apoptosis
Messenger RNA
Propidium
Annexin A5
Multiple Drug Resistance
Skeleton
Human Activities
Ether
Staining and Labeling
Polymerase Chain Reaction
acridone

Keywords

  • Apoptosis
  • Furanoacridones
  • Multidrug resistance

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Antitumour properties of acridone alkaloids on a murine lymphoma cell line. / Réthy, B.; Hohmann, J.; Minorics, Renáta; Varga, András; Ocsovszki, I.; Molnár, J.; Juhász, Kata; Falkay, G.; Zupkó, I.

In: Anticancer Research, Vol. 28, No. 5 A, 09.2008, p. 2737-2743.

Research output: Contribution to journalArticle

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