Antiretroviral effect of 4-thio-uridylate against human immunodeficiency virus type 1

Szilvia Kanizsai, Ágoston Ghidán, Joseph Ongrádi, K. Nagy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Antiretroviral effect of thiolated nucleotide 4-thio-uridylate (S 4 UMP, designated as UD29) against human immunodeficiency virus type 1 (HIV-1) have been quantitatively determined in cell-based viral infectivity assays. In syntitium inhibition assay on MT-2 human T-cell line UD29 prevented cell fusion and formation of syntitia induced by HIVIIIB with IC 50 values of 11.7 μg/ml. In a single-cycle viral infection assay (MAGI assay) UD29 proved to have a potent inhibitory effect against HIV-1 IIIB on HeLaCD4-LTR/β-gal cells, which was dose dependent with IC50 values of 4.75 μg/ml and IC90 of 39.7 μg/ml. UD29 showed a most prominent antiviral effect when administered 30 min prior HIV-1 infection. As HIV entry requires thiol/disulfide exchange process, results suggest that reactive-SH group of enol-form of the thiolated nucleotide may interfere with the function of cell surface proteins. UD29 cannot penetrate into cells and may have an interactive role in redox processes active in viral entry.

Original languageEnglish
Pages (from-to)499-510
Number of pages12
JournalActa Microbiologica et Immunologica Hungarica
Volume59
Issue number4
DOIs
Publication statusPublished - Dec 1 2012

Fingerprint

HIV-1
Virus Diseases
Nucleotides
Uridine Monophosphate
Cell Fusion
Sulfhydryl Compounds
Disulfides
Inhibitory Concentration 50
Oxidation-Reduction
Antiviral Agents
Membrane Proteins
HIV
T-Lymphocytes
Cell Line

Keywords

  • entry inhibitor
  • HIV infection
  • infectivity assays
  • redox system
  • thiolated nucleosides

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Antiretroviral effect of 4-thio-uridylate against human immunodeficiency virus type 1. / Kanizsai, Szilvia; Ghidán, Ágoston; Ongrádi, Joseph; Nagy, K.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 59, No. 4, 01.12.2012, p. 499-510.

Research output: Contribution to journalArticle

Kanizsai, Szilvia ; Ghidán, Ágoston ; Ongrádi, Joseph ; Nagy, K. / Antiretroviral effect of 4-thio-uridylate against human immunodeficiency virus type 1. In: Acta Microbiologica et Immunologica Hungarica. 2012 ; Vol. 59, No. 4. pp. 499-510.
@article{ab1549b8fda348939923e85dcf7fb224,
title = "Antiretroviral effect of 4-thio-uridylate against human immunodeficiency virus type 1",
abstract = "Antiretroviral effect of thiolated nucleotide 4-thio-uridylate (S 4 UMP, designated as UD29) against human immunodeficiency virus type 1 (HIV-1) have been quantitatively determined in cell-based viral infectivity assays. In syntitium inhibition assay on MT-2 human T-cell line UD29 prevented cell fusion and formation of syntitia induced by HIVIIIB with IC 50 values of 11.7 μg/ml. In a single-cycle viral infection assay (MAGI assay) UD29 proved to have a potent inhibitory effect against HIV-1 IIIB on HeLaCD4-LTR/β-gal cells, which was dose dependent with IC50 values of 4.75 μg/ml and IC90 of 39.7 μg/ml. UD29 showed a most prominent antiviral effect when administered 30 min prior HIV-1 infection. As HIV entry requires thiol/disulfide exchange process, results suggest that reactive-SH group of enol-form of the thiolated nucleotide may interfere with the function of cell surface proteins. UD29 cannot penetrate into cells and may have an interactive role in redox processes active in viral entry.",
keywords = "entry inhibitor, HIV infection, infectivity assays, redox system, thiolated nucleosides",
author = "Szilvia Kanizsai and {\'A}goston Ghid{\'a}n and Joseph Ongr{\'a}di and K. Nagy",
year = "2012",
month = "12",
day = "1",
doi = "10.1556/AMicr.59.2012.4.7",
language = "English",
volume = "59",
pages = "499--510",
journal = "Acta Microbiologica et Immunologica Hungarica",
issn = "1217-8950",
publisher = "Akademiai Kiado",
number = "4",

}

TY - JOUR

T1 - Antiretroviral effect of 4-thio-uridylate against human immunodeficiency virus type 1

AU - Kanizsai, Szilvia

AU - Ghidán, Ágoston

AU - Ongrádi, Joseph

AU - Nagy, K.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Antiretroviral effect of thiolated nucleotide 4-thio-uridylate (S 4 UMP, designated as UD29) against human immunodeficiency virus type 1 (HIV-1) have been quantitatively determined in cell-based viral infectivity assays. In syntitium inhibition assay on MT-2 human T-cell line UD29 prevented cell fusion and formation of syntitia induced by HIVIIIB with IC 50 values of 11.7 μg/ml. In a single-cycle viral infection assay (MAGI assay) UD29 proved to have a potent inhibitory effect against HIV-1 IIIB on HeLaCD4-LTR/β-gal cells, which was dose dependent with IC50 values of 4.75 μg/ml and IC90 of 39.7 μg/ml. UD29 showed a most prominent antiviral effect when administered 30 min prior HIV-1 infection. As HIV entry requires thiol/disulfide exchange process, results suggest that reactive-SH group of enol-form of the thiolated nucleotide may interfere with the function of cell surface proteins. UD29 cannot penetrate into cells and may have an interactive role in redox processes active in viral entry.

AB - Antiretroviral effect of thiolated nucleotide 4-thio-uridylate (S 4 UMP, designated as UD29) against human immunodeficiency virus type 1 (HIV-1) have been quantitatively determined in cell-based viral infectivity assays. In syntitium inhibition assay on MT-2 human T-cell line UD29 prevented cell fusion and formation of syntitia induced by HIVIIIB with IC 50 values of 11.7 μg/ml. In a single-cycle viral infection assay (MAGI assay) UD29 proved to have a potent inhibitory effect against HIV-1 IIIB on HeLaCD4-LTR/β-gal cells, which was dose dependent with IC50 values of 4.75 μg/ml and IC90 of 39.7 μg/ml. UD29 showed a most prominent antiviral effect when administered 30 min prior HIV-1 infection. As HIV entry requires thiol/disulfide exchange process, results suggest that reactive-SH group of enol-form of the thiolated nucleotide may interfere with the function of cell surface proteins. UD29 cannot penetrate into cells and may have an interactive role in redox processes active in viral entry.

KW - entry inhibitor

KW - HIV infection

KW - infectivity assays

KW - redox system

KW - thiolated nucleosides

UR - http://www.scopus.com/inward/record.url?scp=84870598510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870598510&partnerID=8YFLogxK

U2 - 10.1556/AMicr.59.2012.4.7

DO - 10.1556/AMicr.59.2012.4.7

M3 - Article

C2 - 23195558

AN - SCOPUS:84870598510

VL - 59

SP - 499

EP - 510

JO - Acta Microbiologica et Immunologica Hungarica

JF - Acta Microbiologica et Immunologica Hungarica

SN - 1217-8950

IS - 4

ER -