Antiproliferative effects of various furanoacridones isolated from ruta graveolens on human breast cancer cell lines

Zsuzsanna Schelz, I. Ocsovszki, Noémi Bózsity, J. Hohmann, I. Zupkó

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background/Aim: Thanks to its biologically active constituents, Ruta graveolens L. (Rutaceae) is a widely used medicinal plant. In our study, six furanoacridone alkaloids isolated from Ruta graveolens were investigated for their antiproliferative and proapoptotic effects on human breast cancer cell lines (MCF-7, MDA-MB-361, MDA-MB-231 and T47D). Materials and Methods: The cell lines were pretreated with alkaloid components (rutacridone, isogravacridone chlorine (IGC), gravacridonediol monomethyl ether, gravacridonediol, gravacridonetriol, a 1:1 mixture of gravacridonetriol and-diol monoglucosides) and their antiproliferative effects were determined by the MTT assay. Results: IGC had the most marked effect on cell proliferation of MDA-MB-231 (half maximal inhibitory concentration (IC50)=2.27 M). Cell-cycle analysis was applied to quantify the effect of IGC on subpopulations of MDA-MB-231 and MCF-7 cells. It caused a cell-cycle disturbance by decreasing the G2/M and G0/G1 and increasing the S phase and the appearance of the subdiploid (sub-G1) population. Hoechst 33258-propidium iodide staining was used to evaluate the morphological changes in IGC-pretreated MDA-MB-231 and MCF-7 cells, revealing the appearance of apoptotic features. IGC was found to cause a modest activation of caspase-3 and-9, but not caspase-8, indicating the activation of an intrinsic apoptotic pathway in MDA-MB-231 cells. Conclusions: These in vitro findings indicate that furanoacridones are suitable candidates for anticancer drug development.

Original languageEnglish
Pages (from-to)2751-2758
Number of pages8
JournalAnticancer Research
Volume36
Issue number6
Publication statusPublished - Jun 1 2016

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Keywords

  • Acridone alkaloids
  • Antitumor effect
  • Apoptosis
  • Furanoacridones
  • Ruta graveolens L.

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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