Antioxidative activity of Allium victorialis L. extracts

Yoshiaki Shirataki, Noboru Motohashi, Satoru Tani, Katsuyoshi Sunaga, Hiroshi Sakagami, Kazue Satoh, Hideki Nakashima, Taisei Kanamoto, Kristina Wolfard, Joseph Molnar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Allium victorialis L. (Liliaceae, "Hon-Gyoujya Nin-Niku" in Japanese) was successively extracted with hexane, acetone, methanol and 70% methanol and the extracts were further separated into a total of twenty-five fractions by silica gel and ODS column chromatographies. The biological activities of these four extracts and 25 column fractions were compared. The cytotoxic activity of all extracts and fractions against two oral tumor cell lines was significantly higher than that against normal human gingival fibroblasts, suggesting their tumor-specific action. Three methanol column fractions [M2, M3, M6] and a 70% methanol column fraction [70M6] most effectively reversed the multidrug resistance (MDR) against L5178 mouse T cell lymphoma. The electron spin resonance (ESR) spectroscopy showed that methanol column fractions and 70% methanol extracts produced the highest amount of radical(s) and most efficiently scavenging O2•-, generated by the hypoxanthine-xanthine reaction system, suggesting that the same substances in these fractions display both prooxidant and antioxidant properties. They showed no anti-human immunodeficiency virus (HIV) or anti-Helicobacter pylori activity. These data suggest the medicinal efficacy of Allium victorialis extract.

Original languageEnglish
Pages (from-to)3331-3339
Number of pages9
JournalAnticancer research
Volume21
Issue number5
Publication statusPublished - Dec 1 2001

Keywords

  • Allium victorialis L. extracts
  • Anti-H. pylori activity
  • Anti-HIV activity
  • Cytotoxic activity
  • MDR reversion
  • O• scavenging activity

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Shirataki, Y., Motohashi, N., Tani, S., Sunaga, K., Sakagami, H., Satoh, K., Nakashima, H., Kanamoto, T., Wolfard, K., & Molnar, J. (2001). Antioxidative activity of Allium victorialis L. extracts. Anticancer research, 21(5), 3331-3339.