Antioxidant status in lung after exposure to cigarette smoke

Zuzana Kováčiková, Elisabeth Tátrai, Milan Beňo, Marta Hurbánková, Silvia Černá, Soňa Wimmerová

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of cigarette smoke exposure on antioxidant status was studied in rats. Rats were exposed for 6 months in an inhalation chamber to cigarette smoke from 3 and 8 cigarettes (1R1) per day, respectively. Glutathione peroxidase, superoxide dismutase, glutathione and ascorbic acid were estimated in lung tissue and bronchoalveolar lavage fluid and compared to control animals. The enzyme activities in lung remained unchanged after the exposure while glutathione was significantly decreased and ascorbic acid increased in the group exposed to the higher cigarette dose. In bronchoalveolar lavage fluid the enzyme activities were decreased while the non-enzymatic antioxidants were not changed. Isolated lung epithelial type II cells showed decrease in both enzyme activities and glutathione after culturing in medium supplemented with cigarette smoke. The results showed high burden of type II cells and different responses of individual antioxidants. The protective role of non-enzymatic antioxidants seems to be dominant.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalBiologia - Section Cellular and Molecular Biology
Volume60
Issue numberSUPPL. 17
Publication statusPublished - Dec 1 2005

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Keywords

  • Antioxidant status
  • Bronchoalveolar lavage fluid
  • Cigarette smoke
  • Lung
  • Lung epithelial type II cells

ASJC Scopus subject areas

  • Genetics
  • Environmental Science(all)
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kováčiková, Z., Tátrai, E., Beňo, M., Hurbánková, M., Černá, S., & Wimmerová, S. (2005). Antioxidant status in lung after exposure to cigarette smoke. Biologia - Section Cellular and Molecular Biology, 60(SUPPL. 17), 85-88.