Antioxidant defence in UV-irradiated tobacco leaves is centred on hydrogen-peroxide neutralization

Petra Majer, Gyula Czégény, Györgyi Sándor, Philip J. Dix, É. Hideg

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Greenhouse grown tobacco (Nicotiana tabacum L. cv. Petit Havana) plants were exposed to supplemental UV centred at 318nm and corresponding to 13.6kJm-2d-1 biologically effective UV-B (280-315nm) radiation. After 6 days this treatment decreased photosynthesis by 30%. Leaves responded by a large increase in UV-absorbing pigment content and antioxidant capacities. UV-stimulated defence against ROS was strongest in chloroplasts, since activities of plastid enzymes FeSOD and APX had larger relative increases than other, non-plastid specific SODs or peroxidases. In addition, non-enzymatic defence against hydroxyl radicals was doubled in UV treated leaves as compared to controls. In UV treated leaves, the extent of activation of ROS neutralizing capacities followed a peroxidases>hydroxyl-radical neutralization>SOD order. These results suggest that highly effective hydrogen peroxide neutralization is the focal point of surviving UV-inducible oxidative stress and argue against a direct signalling role of hydrogen peroxide in maintaining adaptation to UV, at least in laboratory experiments.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalPlant Physiology and Biochemistry
Volume82
DOIs
Publication statusPublished - 2014

Fingerprint

Peroxidases
neutralization
Hydroxyl Radical
Hydrogen Peroxide
Tobacco
hydrogen peroxide
tobacco
Antioxidants
antioxidant activity
peroxidases
hydroxyl radicals
Plastids
superoxide dismutase
Photosynthesis
Chloroplasts
leaves
Oxidative Stress
Radiation
Nicotiana tabacum
plastids

Keywords

  • Antioxidant
  • Hydrogen peroxide
  • Peroxidase
  • Photosynthesis
  • Reactive oxygen species
  • Tobacco
  • UV treatment

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology
  • Medicine(all)

Cite this

Antioxidant defence in UV-irradiated tobacco leaves is centred on hydrogen-peroxide neutralization. / Majer, Petra; Czégény, Gyula; Sándor, Györgyi; Dix, Philip J.; Hideg, É.

In: Plant Physiology and Biochemistry, Vol. 82, 2014, p. 239-243.

Research output: Contribution to journalArticle

Majer, Petra ; Czégény, Gyula ; Sándor, Györgyi ; Dix, Philip J. ; Hideg, É. / Antioxidant defence in UV-irradiated tobacco leaves is centred on hydrogen-peroxide neutralization. In: Plant Physiology and Biochemistry. 2014 ; Vol. 82. pp. 239-243.
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