Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

Anca Hermenean, Miruna Stan, Aurel Ardelean, Luminiţa Pilat, Ciprian Valentin Mihali, Cristina Popescu, Lajos Nagy, G. Deák, M. Zsuga, S. Kéki, I. Bácskay, Ferenc Fenyvesi, Marieta Costache, Anca Dinischiotu, M. Vecsernyés

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalOpen Life Sciences
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Milk Thistle
Silybum marianum
Oilseeds
hepatoprotective effect
seed oils
Seeds
Oils
Antioxidants
antioxidant activity
Oxidative stress
Liver
liver
Oxidative Stress
oxidative stress
Stearic Acids
chloramphenicol acetyltransferase
Tocopherols
peroxidation
mice
Wounds and Injuries

Keywords

  • antioxidant
  • CCl4
  • hepatoprotection
  • oxidative stress
  • seed oil
  • Silybum marianum

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil. / Hermenean, Anca; Stan, Miruna; Ardelean, Aurel; Pilat, Luminiţa; Mihali, Ciprian Valentin; Popescu, Cristina; Nagy, Lajos; Deák, G.; Zsuga, M.; Kéki, S.; Bácskay, I.; Fenyvesi, Ferenc; Costache, Marieta; Dinischiotu, Anca; Vecsernyés, M.

In: Open Life Sciences, Vol. 10, No. 1, 01.01.2015, p. 147-158.

Research output: Contribution to journalArticle

Hermenean, A, Stan, M, Ardelean, A, Pilat, L, Mihali, CV, Popescu, C, Nagy, L, Deák, G, Zsuga, M, Kéki, S, Bácskay, I, Fenyvesi, F, Costache, M, Dinischiotu, A & Vecsernyés, M 2015, 'Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil', Open Life Sciences, vol. 10, no. 1, pp. 147-158. https://doi.org/10.1515/biol-2015-0017
Hermenean, Anca ; Stan, Miruna ; Ardelean, Aurel ; Pilat, Luminiţa ; Mihali, Ciprian Valentin ; Popescu, Cristina ; Nagy, Lajos ; Deák, G. ; Zsuga, M. ; Kéki, S. ; Bácskay, I. ; Fenyvesi, Ferenc ; Costache, Marieta ; Dinischiotu, Anca ; Vecsernyés, M. / Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil. In: Open Life Sciences. 2015 ; Vol. 10, No. 1. pp. 147-158.
@article{e029334d30814c96a0434eaaf5c1cb87,
title = "Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil",
abstract = "This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50{\%} v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.",
keywords = "antioxidant, CCl4, hepatoprotection, oxidative stress, seed oil, Silybum marianum",
author = "Anca Hermenean and Miruna Stan and Aurel Ardelean and Luminiţa Pilat and Mihali, {Ciprian Valentin} and Cristina Popescu and Lajos Nagy and G. De{\'a}k and M. Zsuga and S. K{\'e}ki and I. B{\'a}cskay and Ferenc Fenyvesi and Marieta Costache and Anca Dinischiotu and M. Vecserny{\'e}s",
year = "2015",
month = "1",
day = "1",
doi = "10.1515/biol-2015-0017",
language = "English",
volume = "10",
pages = "147--158",
journal = "Open Life Sciences",
issn = "1895-104X",
publisher = "Walter de Gruyter GmbH",
number = "1",

}

TY - JOUR

T1 - Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

AU - Hermenean, Anca

AU - Stan, Miruna

AU - Ardelean, Aurel

AU - Pilat, Luminiţa

AU - Mihali, Ciprian Valentin

AU - Popescu, Cristina

AU - Nagy, Lajos

AU - Deák, G.

AU - Zsuga, M.

AU - Kéki, S.

AU - Bácskay, I.

AU - Fenyvesi, Ferenc

AU - Costache, Marieta

AU - Dinischiotu, Anca

AU - Vecsernyés, M.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.

AB - This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.

KW - antioxidant

KW - CCl4

KW - hepatoprotection

KW - oxidative stress

KW - seed oil

KW - Silybum marianum

UR - http://www.scopus.com/inward/record.url?scp=84927934914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927934914&partnerID=8YFLogxK

U2 - 10.1515/biol-2015-0017

DO - 10.1515/biol-2015-0017

M3 - Article

AN - SCOPUS:84927934914

VL - 10

SP - 147

EP - 158

JO - Open Life Sciences

JF - Open Life Sciences

SN - 1895-104X

IS - 1

ER -