Abstract
This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.
| Original language | English |
|---|---|
| Pages (from-to) | 147-158 |
| Number of pages | 12 |
| Journal | Open Life Sciences |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2015 |
Fingerprint
Keywords
- antioxidant
- CCl4
- hepatoprotection
- oxidative stress
- seed oil
- Silybum marianum
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Neuroscience(all)
Cite this
Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil. / Hermenean, Anca; Stan, Miruna; Ardelean, Aurel; Pilat, Luminiţa; Mihali, Ciprian Valentin; Popescu, Cristina; Nagy, Lajos; Deák, G.; Zsuga, M.; Kéki, S.; Bácskay, I.; Fenyvesi, Ferenc; Costache, Marieta; Dinischiotu, Anca; Vecsernyés, M.
In: Open Life Sciences, Vol. 10, No. 1, 01.01.2015, p. 147-158.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil
AU - Hermenean, Anca
AU - Stan, Miruna
AU - Ardelean, Aurel
AU - Pilat, Luminiţa
AU - Mihali, Ciprian Valentin
AU - Popescu, Cristina
AU - Nagy, Lajos
AU - Deák, G.
AU - Zsuga, M.
AU - Kéki, S.
AU - Bácskay, I.
AU - Fenyvesi, Ferenc
AU - Costache, Marieta
AU - Dinischiotu, Anca
AU - Vecsernyés, M.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.
AB - This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.
KW - antioxidant
KW - CCl4
KW - hepatoprotection
KW - oxidative stress
KW - seed oil
KW - Silybum marianum
UR - http://www.scopus.com/inward/record.url?scp=84927934914&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84927934914&partnerID=8YFLogxK
U2 - 10.1515/biol-2015-0017
DO - 10.1515/biol-2015-0017
M3 - Article
AN - SCOPUS:84927934914
VL - 10
SP - 147
EP - 158
JO - Open Life Sciences
JF - Open Life Sciences
SN - 1895-104X
IS - 1
ER -