Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth

Tomislav Meštrović, D. Virók, Sunčanica Ljubin-Sternak, Tímea Raffai, K. Burián, Jasmina Vraneš

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Obligate intracellular localization of Chlamydia trachomatis (C. trachomatis) complicates antimicrobial sensitivity testing efforts that we are so accustomed to in routine bacteriology. Cell culture systems with immunofluorescence staining, to identify cellular inclusions in the presence of various concentrations of antimicrobial drugs, are still the most pervasive techniques, but more specific and sensitive nucleic acid concentration measuring methods are increasingly being used. Here we describe how to approach antimicrobial susceptibility/resistance screening in C. trachomatis by using a McCoy cell culture system, optimized by a research group from Croatia, and direct qPCR-based monitoring of chlamydial growth, optimized by a research group from Hungary.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages33-43
Number of pages11
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2042
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Chlamydia trachomatis
Cell Culture Techniques
Croatia
Bacteriology
Hungary
Inclusion Bodies
Growth
Research
Nucleic Acids
Fluorescent Antibody Technique
Staining and Labeling
Pharmaceutical Preparations

Keywords

  • Antimicrobial resistance
  • Antimicrobial susceptibility testing
  • Cell culture
  • Chlamydia trachomatis
  • qPCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Meštrović, T., Virók, D., Ljubin-Sternak, S., Raffai, T., Burián, K., & Vraneš, J. (2019). Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth. In Methods in Molecular Biology (pp. 33-43). (Methods in Molecular Biology; Vol. 2042). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9694-0_5

Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth. / Meštrović, Tomislav; Virók, D.; Ljubin-Sternak, Sunčanica; Raffai, Tímea; Burián, K.; Vraneš, Jasmina.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 33-43 (Methods in Molecular Biology; Vol. 2042).

Research output: Chapter in Book/Report/Conference proceedingChapter

Meštrović, T, Virók, D, Ljubin-Sternak, S, Raffai, T, Burián, K & Vraneš, J 2019, Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2042, Humana Press Inc., pp. 33-43. https://doi.org/10.1007/978-1-4939-9694-0_5
Meštrović T, Virók D, Ljubin-Sternak S, Raffai T, Burián K, Vraneš J. Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 33-43. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9694-0_5
Meštrović, Tomislav ; Virók, D. ; Ljubin-Sternak, Sunčanica ; Raffai, Tímea ; Burián, K. ; Vraneš, Jasmina. / Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 33-43 (Methods in Molecular Biology).
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