Antimicrobial nodule-specific cysteine-rich peptides disturb the integrity of bacterial outer and inner membranes and cause loss of membrane potential

Kata R. Mikuláss, Krisztina Nagy, Balázs Bogos, Zsolt Szegletes, Etelka Kovács, Attila Farkas, György Váró, Éva Kondorosi, Attila Kereszt

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Certain legume plants produce a plethora of AMP-like peptides in their symbiotic cells. The cationic subgroup of the nodule-specific cysteine-rich (NCR) peptides has potent antimicrobial activity against gram-negative and gram-positive bacteria as well as unicellular and filamentous fungi. Findings: It was shown by scanning and atomic force microscopies that the cationic peptides NCR335, NCR247 and Polymyxin B (PMB) affect differentially on the surfaces of Sinorhizobium meliloti bacteria. Similarly to PMB, both NCR peptides caused damages of the outer and inner membranes but at different extent and resulted in the loss of membrane potential that could be the primary reason of their antimicrobial activity. Conclusions: The primary reason for bacterial cell death upon treatment with cationic NCR peptides is the loss of membrane potential.

Original languageEnglish
Article number43
JournalAnnals of Clinical Microbiology and Antimicrobials
Volume15
Issue number1
DOIs
Publication statusPublished - Jul 28 2016

Keywords

  • Antimicrobial activity
  • Atomic force and scanning electron microscopy
  • Membrane disruption

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Antimicrobial nodule-specific cysteine-rich peptides disturb the integrity of bacterial outer and inner membranes and cause loss of membrane potential'. Together they form a unique fingerprint.

  • Cite this