Antimicrobial and antioxidant activity of kaempferol rhamnoside derivatives from Bryophyllum pinnatum

Simplice Joel Ndendoung Tatsimo, Jean De Dieu Tamokou, Léopold Havyarimana, Dezs Csupor, P. Forgó, J. Hohmann, Jules Roger Kuiate, Pierre Tane

Research output: Contribution to journalArticle

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Abstract

Background: Bryophyllum pinnatum (Lank.) Oken (Crassulaceae) is a perennial succulent herb widely used in traditional medicine to treat many ailments. Its wide range of uses in folk medicine justifies its being called "life plant" or "resurrection plant", prompting researchers' interest. We describe here the isolation and structure elucidation of antimicrobial and/or antioxidant components from the EtOAc extract of B. pinnatum. Results: The methanol extract displayed both antimicrobial activities with minimum inhibitory concentration (MIC) values ranging from 32 to 512 μg/ml and antioxidant property with an IC 50value of 52.48 μg/ml. Its partition enhanced the antimicrobial activity in EtOAc extract (MIC = 16-128 μg/ml) and reduced it in hexane extract (MIC = 256-1024 μg/ml). In addition, this process reduced the antioxidant activity in EtOAc and hexane extracts with IC 50values of 78.11 and 90.04 μg/ml respectively. Fractionation of EtOAc extract gave seven kaempferol rhamnosides, including; kaempferitrin (1), kaempferol 3-O-α-L-(2-acetyl)rhamnopyranoside-7-O-α-L- rhamnopyranoside (2), kaempferol 3-O-α-L-(3-acetyl)rhamnopyranoside-7-O- α-L-rhamnopyranoside (3), kaempferol 3-O-α-L-(4-acetyl) rhamnopyranoside-7-O-α-L-rhamnopyranoside (4), kaempferol 3-O-α-D- glucopyranoside-7-O-α-L-rhamnopyranoside (5), afzelin (6) and α-rhamnoisorobin (7). All these compounds, except 6 were isolated from this plant for the first time. Compound 7 was the most active, with MIC values ranging from 1 to 2 μg/ml and its antioxidant activity (IC 50= 0.71 μg/ml) was higher than that of the reference drug (IC 50= 0.96 μg/ml). Conclusion: These findings demonstrate that Bryophyllum pinnatum and some of its isolated compounds have interesting antimicrobial and antioxidant properties, and therefore confirming the traditional use of B. pinnatum in the treatment of infectious and free radical damages.

Original languageEnglish
Article number158
JournalBMC Research Notes
Volume5
DOIs
Publication statusPublished - 2012

Fingerprint

Kalanchoe
Microbial Sensitivity Tests
Antioxidants
Derivatives
Hexanes
Traditional Medicine
Medicine
Craterostigma
Crassulaceae
Fractionation
Free Radicals
Methanol
Research Personnel
kaempferol
Pharmaceutical Preparations

Keywords

  • Antimicrobial
  • Antioxidant
  • Bryophyllum pinnatum
  • Crassulaceae
  • Kaempferol rhamnosides
  • Minimum inhibitory concentration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Antimicrobial and antioxidant activity of kaempferol rhamnoside derivatives from Bryophyllum pinnatum. / Tatsimo, Simplice Joel Ndendoung; Tamokou, Jean De Dieu; Havyarimana, Léopold; Csupor, Dezs; Forgó, P.; Hohmann, J.; Kuiate, Jules Roger; Tane, Pierre.

In: BMC Research Notes, Vol. 5, 158, 2012.

Research output: Contribution to journalArticle

Tatsimo, Simplice Joel Ndendoung ; Tamokou, Jean De Dieu ; Havyarimana, Léopold ; Csupor, Dezs ; Forgó, P. ; Hohmann, J. ; Kuiate, Jules Roger ; Tane, Pierre. / Antimicrobial and antioxidant activity of kaempferol rhamnoside derivatives from Bryophyllum pinnatum. In: BMC Research Notes. 2012 ; Vol. 5.
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abstract = "Background: Bryophyllum pinnatum (Lank.) Oken (Crassulaceae) is a perennial succulent herb widely used in traditional medicine to treat many ailments. Its wide range of uses in folk medicine justifies its being called {"}life plant{"} or {"}resurrection plant{"}, prompting researchers' interest. We describe here the isolation and structure elucidation of antimicrobial and/or antioxidant components from the EtOAc extract of B. pinnatum. Results: The methanol extract displayed both antimicrobial activities with minimum inhibitory concentration (MIC) values ranging from 32 to 512 μg/ml and antioxidant property with an IC 50value of 52.48 μg/ml. Its partition enhanced the antimicrobial activity in EtOAc extract (MIC = 16-128 μg/ml) and reduced it in hexane extract (MIC = 256-1024 μg/ml). In addition, this process reduced the antioxidant activity in EtOAc and hexane extracts with IC 50values of 78.11 and 90.04 μg/ml respectively. Fractionation of EtOAc extract gave seven kaempferol rhamnosides, including; kaempferitrin (1), kaempferol 3-O-α-L-(2-acetyl)rhamnopyranoside-7-O-α-L- rhamnopyranoside (2), kaempferol 3-O-α-L-(3-acetyl)rhamnopyranoside-7-O- α-L-rhamnopyranoside (3), kaempferol 3-O-α-L-(4-acetyl) rhamnopyranoside-7-O-α-L-rhamnopyranoside (4), kaempferol 3-O-α-D- glucopyranoside-7-O-α-L-rhamnopyranoside (5), afzelin (6) and α-rhamnoisorobin (7). All these compounds, except 6 were isolated from this plant for the first time. Compound 7 was the most active, with MIC values ranging from 1 to 2 μg/ml and its antioxidant activity (IC 50= 0.71 μg/ml) was higher than that of the reference drug (IC 50= 0.96 μg/ml). Conclusion: These findings demonstrate that Bryophyllum pinnatum and some of its isolated compounds have interesting antimicrobial and antioxidant properties, and therefore confirming the traditional use of B. pinnatum in the treatment of infectious and free radical damages.",
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AU - Tamokou, Jean De Dieu

AU - Havyarimana, Léopold

AU - Csupor, Dezs

AU - Forgó, P.

AU - Hohmann, J.

AU - Kuiate, Jules Roger

AU - Tane, Pierre

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N2 - Background: Bryophyllum pinnatum (Lank.) Oken (Crassulaceae) is a perennial succulent herb widely used in traditional medicine to treat many ailments. Its wide range of uses in folk medicine justifies its being called "life plant" or "resurrection plant", prompting researchers' interest. We describe here the isolation and structure elucidation of antimicrobial and/or antioxidant components from the EtOAc extract of B. pinnatum. Results: The methanol extract displayed both antimicrobial activities with minimum inhibitory concentration (MIC) values ranging from 32 to 512 μg/ml and antioxidant property with an IC 50value of 52.48 μg/ml. Its partition enhanced the antimicrobial activity in EtOAc extract (MIC = 16-128 μg/ml) and reduced it in hexane extract (MIC = 256-1024 μg/ml). In addition, this process reduced the antioxidant activity in EtOAc and hexane extracts with IC 50values of 78.11 and 90.04 μg/ml respectively. Fractionation of EtOAc extract gave seven kaempferol rhamnosides, including; kaempferitrin (1), kaempferol 3-O-α-L-(2-acetyl)rhamnopyranoside-7-O-α-L- rhamnopyranoside (2), kaempferol 3-O-α-L-(3-acetyl)rhamnopyranoside-7-O- α-L-rhamnopyranoside (3), kaempferol 3-O-α-L-(4-acetyl) rhamnopyranoside-7-O-α-L-rhamnopyranoside (4), kaempferol 3-O-α-D- glucopyranoside-7-O-α-L-rhamnopyranoside (5), afzelin (6) and α-rhamnoisorobin (7). All these compounds, except 6 were isolated from this plant for the first time. Compound 7 was the most active, with MIC values ranging from 1 to 2 μg/ml and its antioxidant activity (IC 50= 0.71 μg/ml) was higher than that of the reference drug (IC 50= 0.96 μg/ml). Conclusion: These findings demonstrate that Bryophyllum pinnatum and some of its isolated compounds have interesting antimicrobial and antioxidant properties, and therefore confirming the traditional use of B. pinnatum in the treatment of infectious and free radical damages.

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KW - Antioxidant

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KW - Kaempferol rhamnosides

KW - Minimum inhibitory concentration

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