Antigenic relationship between human and animal adenovirus hexons determined by means of monoclonal antibodies directed against bovine adenovirus type 2 hexon

Éva Ádám, M. Rusvai, Anna Lengyel, S. Belák, I. Nász

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Eight monoclonal antibodies (MAbs) directed against bovine adenovirus (BAV) 2 subtype B hexon were studied with 12 different hexon types of human adenoviruses (AV) belonging to 5 different subgenera using indirect ELISA, passive hemagglutination (HA), and gel diffusion assays. Two hexon types of animal origin (BAV3 and SAV16) were investigated, too. The reactivity of the MAb IV.F3 was the broadest, i.e. in ELISA and HA experiments it reacted with all hexon types studied. Based on these results as well as on the results of gel diffusion assays, this MAb should recognize the genus specific epitope of adenovirus hexons. Three MAbs (CA12, III.B11, and A12) could recognize different epitopes showing intersubgenus or intertype specificities. In spite of the fact, that all the eight MAbs proved to be bound by the hexon of ORT/111 (BAV2 subtype B) blotted onto nitrocellulose filter, four of the eight MAbs (BB7, BH5, II.A9, and IV.F5) failed to react with any human, and animal hexon types used in the present experiments. The results suggest that a gradient of antigenic relationship may exist between BAV2 hexon and the hexons of human serotypes.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalArchives of Virology
Volume100
Issue number1-2
DOIs
Publication statusPublished - Mar 1988

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Human Adenoviruses
Adenoviridae
Monoclonal Antibodies
Hemagglutination
Epitopes
Gels
Enzyme-Linked Immunosorbent Assay
compound A 12
Collodion

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Antigenic relationship between human and animal adenovirus hexons determined by means of monoclonal antibodies directed against bovine adenovirus type 2 hexon. / Ádám, Éva; Rusvai, M.; Lengyel, Anna; Belák, S.; Nász, I.

In: Archives of Virology, Vol. 100, No. 1-2, 03.1988, p. 9-15.

Research output: Contribution to journalArticle

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